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A rapid fluorescence based method for the quantitative analysis of cell culture media photo-degradation
(Elsevier (Science Direct), 2013-11-21)
Cell culture media are very complex chemical mixtures that are one of the most important aspects in biopharmaceutical manufacturing. The complex composition of many media leads to materials that are inherently unstable and ...
Quantitative analysis of cocaine in solid mixtures using Raman spectroscopy and chemometric methods
(Wiley, 2000-04-27)
Near-infrared (785 mn) excitation was used to obtain Raman spectra from a series of 33 solid mixtures containing cocaine, caffeine and glucose (9.8-80.6% by weight cocaine), which were then analysed using chemometric ...
Using polarized Total Synchronous Fluorescence Spectroscopy (pTSFS) with PARAFAC analysis for characterizing intrinsic protein emission
(Elsevier, 2019-10-15)
Using polarized Excitation Emission Matrix (pEEM) spectroscopy to measure the intrinsic emission of proteins offers a potentially useful methodology for a wide variety of potential applications. However, the presence of ...
Development of a rapid Polarized Total Synchronous Fluorescence Spectroscopy (pTSFS) method for protein quantification in a model bioreactor broth
(Wiley, 2021-01-27)
Protein quantification during bioprocess monitoring is essential for biopharmaceutical manufacturing and is complicated by the complex chemical composition of the bioreactor broth. Here we present the early-stage development ...
Evaluating the interaction of human serum albumin (HSA) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes in different aqueous environments using anisotropy resolved multi-dimensional emission spectroscopy (ARMES)
(Elsevier, 2022-01-07)
Studying the interaction between plasma proteins and liposomes is critical, particularly for their use as drug delivery systems. Here, the efficacy of anisotropy resolved multidimensional emission spectroscopy (ARMES) for ...
Modelling Förster resonance energy transfer (FRET) using anisotropy resolved multi-dimensional emission spectroscopy (ARMES)
(Elsevier, 2020-11-16)
Background
Förster Resonance Energy Transfer (FRET) is widely used to study the structure and dynamics of biomolecular systems and also causes the non-linear fluorescence response observed in multi-fluorophore proteins. ...
Analyzing protein conjugation reactions for antibody-drug conjugate synthesis using polarized excitation emission matrix spectroscopy
(Wiley, 2022-09-07)
Antibody-drug conjugates (ADCs) are promising anticancer therapeutics, which offer important advantages compared to more classical therapies. There are a variety of ADC critical quality attributes (CQAs) such as the protein ...
Size exclusion chromatography for screening yeastolate used in cell culture media
(Elsevier, 2023-09-18)
Yeastolate is often used as a media supplement in industrial mammalian cell culture or as a major media component for microbial fermentations. Yeastolate variability can significantly affect process performance, but analysis ...