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Novel Multiplex Real-Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains
(Journal of Clinical Microbiology, 2011-02)
Tuberculosis (TB) in humans is caused by members of the Mycobacterium tuberculosis complex (MTC). Rapid detection of the MTC is necessary for the timely initiation of antibiotic treatment, while differentiation between ...
Comparative genome analysis identifies novel nucleic acid diagnostic targets for use in the specific detection of Haemophilus influenzae
(Elsevier, 2015-06-23)
Haemophilus influenzae is recognised as an important human pathogen associated with invasive infections, including bloodstream infection and meningitis. Currently used molecular-based diagnostic assays lack specificity in ...
A rapid culture independent methodology to quantitatively detect and identify common human bacterial pathogens associated with contaminated high purity water
(BioMed Central, 2015-02-18)
Water and High Purity Water (HPW) distribution systems can be contaminated with human
pathogenic microorganisms. This biocontamination may pose a risk to human health as HPW is commonly used in
the industrial, pharmaceutical ...
Comparison of established diagnostic methodologies and a novel bacterial smpB real-time PCR assay for the specific detection of haemophilus influenzae associated with respiratory tract infections
(American Society for Microbiology, 2015-06-23)
Haemophilus influenzae is a significant causative agent of respiratory tract infections (RTI) worldwide. The development of a
rapid H. influenzae diagnostic assay that would allow for the implementation of infection control ...
Low-temperature anaerobic digestion is associated with differential methanogenic protein expression
(Oxford University Press, 2015-04-09)
Anaerobic digestion (AD) is an attractive wastewater treatment technology, leading to the generation of recoverable biofuel (methane). Most industrial AD applications, carry excessive heating costs, however, as AD reactors ...
Cross platform standardisation of an experimental pipeline for use in the identification of dysregulated human circulating MiRNAs
(Public Library of Science, 2015-09-10)
Micro RNAs (miRNAs) are a class of highly conserved small non-coding RNAs that play an important part in the post-transcriptional regulation of gene expression. A substantial number of miRNAs have been proposed as biomarkers ...
Determination of survival of wildtype and mutant Escherichia coli in soil
(Bio-protocol, 2017-07-20)
E. coli resides in the gastrointestinal tract of humans and other warm-blooded animals but recent studies have shown that E. coli can persist and grow in various external environments including soil. The general stress ...
The Vibrio parahaemolyticus Type III Secretion Systems manipulate host cell MAPK for critical steps in pathogenesis
(BioMed Central, 2011)
Background:Vibrio parahaemolyticus is a food-borne pathogen causing inflammation of the gastrointestinal epithelium. Pathogenic strains of this bacterium possess two Type III Secretion Systems (TTSS) that deliver effector ...
Temperature-dependent phenotypic variation of Campylobacter jejuni lipooligosaccharides
(2010-11)
Background:Campylobacter jejuni is a major bacterial cause of food-borne enteritis, and its lipooligosaccharide (LOS) plays an initiating role in the development of the autoimmune neuropathy, Guillain-Barré syndrome, by ...