Browsing Microbiology (Scholarly Articles) by Issue Date
Now showing items 1-20 of 65
-
The isolation of strains of Bacillus subtilis showing improved plasmid stability characteristics by means of selective chemostat culture
(1988-03-23)A PUB 110-derived plasmid encoding chloramphenicol resistance, kanamycin resistance and high-temperature alpha-amylase showed a high degree of segregational instability when inserted into Bacillus subtilis. In an attempt ... -
Role of alginate lyase in cell detachment of Pseudomonas aeruginosa
(American Society for Microbiology, 1994-07)The exopolysaccharide alginate of Pseudomonas aeruginosa was shown to be important in determining the degree of cell detachment from an agar surface. Nonmucoid strain 8822 gave rise to 50-fold more sloughed cells than ... -
LcrG is required for efficient translocation of Yersinia Yop effector proteins into eukaryotic cells
(American Society for Microbiology, 1998-06)Extracellular Yersinia disables the immune system of its host by injecting effector Yop proteins into host cells. We show that a Yersinia enterocolitica nonpolar lcrG mutant is severely impaired in the translocation of ... -
The virulence plasmid of Yersinia, an anti-host genome
(American Society for Microbiology, 1998-12)Invasive pathogenic bacteria have in common the capacity to overcome the defense mechanisms of their animal host and to proliferate in its tissues. They each have their own life-style and target organs and cause a variety ... -
Competition between the Yops of Yersinia enterocolitica for delivery into eukaryotic cells: Role of the SycE chaperone binding domain of YopE
(American Society for Microbiology, 2000-09)A type III secretion-translocation system allows Yersinia adhering at the surface of animal cells to deliver a cocktail of effector Yops (YopH, -O, -P, -E, -M, and -T) into the cytosol of these cells. Residues or codons 1 ... -
Adenylate Cyclase Toxin from Bordetella pertussis Synergizes with Lipopolysaccharide To Promote Innate Interleukin-10 Production and Enhances the Induction of Th2 and Regulatory T Cells
(American Society for Microbiology, 2004-03)Adenylate cyclase toxin (CyaA) from Bordetella pertussis can subvert host immune responses allowing bacterial colonization. Here we have examined its adjuvant and immunomodulatory properties and the possible contribution ... -
Bordetella pertussis expresses a functional type III secretion system that subverts protective innate and adaptive immune responses
(American Society for Microbiology, 2008-01)Certain bacteria use a type III secretion system (TTSS) to deliver effector proteins that interfere with cell function into host cells. While transcription of genes encoding TTSS components has been demonstrated, studies ... -
Identification of Components of the Sigma B Regulon in Listeria monocytogenes That Contribute to Acid and Salt Tolerance
(American Society for Microbiology, 2008-09)Sigma B is an alternative sigma factor that controls the transcriptional response to stress in Listeria monocytogenes and is also known to play a role in the virulence of this human pathogen. In the present study we ... -
Specificity and sensitivity evaluation of novel and existing Bacteroidales and Bifidobacteria-specific PCR assays on feces and sewage samples and their application for microbial source tracking in Ireland
(Elsevier, 2009)Three novel ruminant-specific PCR assays, an existing ruminant-specific PCR assay and five existing human-specific PCR assays, which target 16S rDNA from Bacteroidales or Bifidobacteria, were evaluated. The assays were ... -
Control of transfer and weak closure in fusion systems
(2010)We show that K¿ and K¿ control transfer in every fusion system on a finite p-group when p ¿ 5, and that they control weak closure of elements in every fusion system on a finite p-group when p ¿ 3. This generalizes resultsof ... -
Intracellular accumulation of high levels of gamma-aminobutyrate by Listeria monocytogenes 10403S in response to low pH: uncoupling of gamma-aminobutyrate synthesis from efflux in a chemically defined medium
(American Society for Microbiology, 2010-06)It is well established that the glutamate decarboxylase (GAD) system is central to the survival of Listeria monocytogenes at low pH, both in acidic foods and within the mammalian stomach. The accepted model proposes that ... -
Temperature-dependent phenotypic variation of Campylobacter jejuni lipooligosaccharides
(2010-11)Background:Campylobacter jejuni is a major bacterial cause of food-borne enteritis, and its lipooligosaccharide (LOS) plays an initiating role in the development of the autoimmune neuropathy, Guillain-Barré syndrome, by ... -
The Vibrio parahaemolyticus Type III Secretion Systems manipulate host cell MAPK for critical steps in pathogenesis
(BioMed Central, 2011)Background:Vibrio parahaemolyticus is a food-borne pathogen causing inflammation of the gastrointestinal epithelium. Pathogenic strains of this bacterium possess two Type III Secretion Systems (TTSS) that deliver effector ... -
Novel Multiplex Real-Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains
(Journal of Clinical Microbiology, 2011-02)Tuberculosis (TB) in humans is caused by members of the Mycobacterium tuberculosis complex (MTC). Rapid detection of the MTC is necessary for the timely initiation of antibiotic treatment, while differentiation between ... -
A Novel Multiplex Real-Time PCR for the Identification of Mycobacteria Associated with Zoonotic Tuberculosis
(PLoS ONE, 2011-08-09)Background: Tuberculosis (TB) is the leading cause of death worldwide from a single infectious agent. An ability to detect the Mycobacterium tuberculosis complex (MTC) in clinical material while simultaneously differentiating ... -
The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model
(American Society for Microbiology, 2012-03)Vibrio parahaemolyticus, a marine bacterium, is the causative agent of gastroenteritis associated with the consumption of seafood. It contains a homologue of the toxRS operon that in V. cholerae is the key regulator of ... -
Impact of trichloroethylene exposure on the microbial diversity and protein expression in anaerobic granular biomass at 37°C and 15°C
(Hindawi Publishing Corporation, 2012-09-14)Granular biomass from a laboratory-scale anaerobic bioreactor trial was analysed to identify changes in microbial community structure and function in response to temperature and trichloroethylene (TCE). Two bioreactors ... -
Listeria monocytogenes has a functional gamma-aminobutyrate (GABA) shunt: Role in acid tolerance and succinate biosynthesis.
(American Society for Microbiology, 2013)Listeria monocytogenes, the causative agent of human listeriosis, is known for its ability to withstand severe environmental stresses. The glutamate decarboxylase (GAD) system is one of the principal systems utilized by ... -
Low-temperature (10°C) anaerobic digestion of dilute dairy wastewater in an EGSB bioreactor: microbial community structure, population dynamics, and kinetics of methanogenic populations
(Hindawi Publishing Corporation, 2013-07-25)The feasibility of anaerobic digestion of dairy wastewater at 10 degrees C was investigated in a high height : diameter ratio EGSB reactor. Stable performance was observed at an applied organic loading rate (OLR) of 0.5-2 ... -
Contrasting responses to nutrient enrichment of prokaryotic communities collected from deep sea sites in the Southern Ocean
(MDPI Open-Access Journals, 2013-09-13)Abstract: Deep water samples (ca. 4,200 m) were taken from two hydrologically-similar sites around the Crozet islands with highly contrasting surface water productivities. Site M5 was characteristic of high productivity ...