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dc.contributor.authorReddington, Kate
dc.contributor.authorO'Grady, Justin
dc.contributor.authorDorai-Raj, Siobhan
dc.contributor.authorMaher, Majella
dc.contributor.authorvan Soolingen, Dick
dc.contributor.authorBarry, Thomas
dc.date.accessioned2012-02-13T13:44:30Z
dc.date.available2012-02-13T13:44:30Z
dc.date.issued2011-02
dc.identifier.citationReddington, K., J. O'Grady, S. Dorai-Raj, M. Maher, D. van Soolingen, and T. Barry. 2011. Novel Multiplex Real-Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains. J. Clin. Microbiol. 49:651-657en_US
dc.identifier.urihttp://hdl.handle.net/10379/2563
dc.description.abstractTuberculosis (TB) in humans is caused by members of the Mycobacterium tuberculosis complex (MTC). Rapid detection of the MTC is necessary for the timely initiation of antibiotic treatment, while differentiation between members of the complex may be important to guide the appropriate antibiotic treatment and provide epidemiological information. In this study, a multiplex real-time PCR diagnostics assay using novel molecular targets was designed to identify the MTC while simultaneously differentiating between M. tuberculosis and M. canettii. The lepA gene was targeted for the detection of members of the MTC, the wbbl1 gene was used for the differentiation of M. tuberculosis and M. canettii from the remainder of the complex, and a unique region of the M. canettii genome, a possible novel region of difference (RD), was targeted for the specific identification of M. canettii. The multiplex real-time PCR assay was tested using 125 bacterial strains (64 MTC isolates, 44 nontuberculosis mycobacteria [NTM], and 17 other bacteria). The assay was determined to be 100% specific for the mycobacteria tested. Limits of detection of 2.2, 2.17, and 0.73 cell equivalents were determined for M. tuberculosis/M. canettii, the MTC, and M. canettii, respectively, using probit regression analysis. Further validation of this diagnostics assay, using clinical samples, should demonstrate its potential for the rapid, accurate, and sensitive diagnosis of TB caused by M. tuberculosis, M. canettii, and the other members of the MTC.en_US
dc.formatdoi:10.1128/JCM.01426-10en_US
dc.language.isoenen_US
dc.publisherJournal of Clinical Microbiologyen_US
dc.subjectTuberculosisen_US
dc.subjectMycobacterium tuberculosis complex (MTC)en_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectMycobacterium canettiien_US
dc.subjectMicrobiologyen_US
dc.titleNovel Multiplex Real-Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strainsen_US
dc.typeArticleen_US
dc.local.publishedsourcehttp://jcm.asm.org/content/49/2/651.full.pdf+htmlen_US
dc.description.peer-reviewedpeer-revieweden_US
dc.contributor.funderCollege of Sciences, National University of Ireland Galway funded KR Postgraduate Scholarshipen_US
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