Browsing National Centre for Biomedical Engineering Science (Scholarly Articles) by Issue Date
Now showing items 1-20 of 79
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Evaluation of a novel PCR based diagnostic assay for the detection of Mycobacterium tuberculosis in sputum specimens
(ACM, 1996-06-18)We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were ... -
Human chorionic gonadotrophin relaxation of human pregnant myometrium and activation of the BKCa channel
(2003)The uterorelaxant effect of human chorionic gonadotropin (hCG) is regarded as an important mediator in maintenance of uterine quiescence during pregnancy with clinical potential for tocolysis, the mechanisms of which are ... -
Gene prediction using the Self-Organizing Map: automatic generation of multiple gene models
(2004-03-05)This work explores a new approach to gene-prediction, based on the Self-Organizing Map, which has the ability to automatically identify multiple gene models within a genome. The current implementation, named RescueNet, ... -
Analysis of Maxi-K alpha subunit splice variants in human myometrium
(springer, 2004-09-21)Background: Large-conductance, calcium-activated potassium (Maxi-K) channels are implicated in the modulation of human uterine contractions and myometrial Ca2+ homeostasis. However, the regulatory mechanism(s) governing ... -
Isolation and characterization of the bovine Stearoyl-CoAdesaturase promoter and analysis of polymorphisms in the promoter region in dairy cows
(Springer, 2005-03)Conjugated linoleic acid (CLA) in milk arises through microbial biohydrogenation of dietary polyunsaturated fatty acids (PUFA) in the rumen, and by the action of mammary Stearoyl-CoA desaturase (Scd). A large variation ... -
Gene prediction using the Self-Organizing Map: automatic generation of multiple gene models
(BioMed Central, 2005-03-05)Background: Many current gene prediction methods use only one model to represent protein-coding regions in a genome, and so are less likely to predict the location of genes that have an atypical sequence composition. It ... -
Transcription factor binding site identification using the Self-Organizing Map
(Oxford Open Journals, 2005-05-01)Motivation: The automatic identification of over-represented motifs present in a collection of sequences continues to be a challenging problem in computational biology. In this paper, we propose a self-organizing map of ... -
Functional coupling of beta(3)-adrenoceptors and large conductance calcium-activated potassium channels in human uterine myocytes
(Endocrine Society, 2005-07-02)Context: beta(3)-Adrenoreceptor modulation in human myometrium during pregnancy is linked functionally to myometrial inhibition. Maxi-K+ channels (BKCa) play a significant role in modulating cell membrane potential and ... -
A single nucleotide polymorphism in the bovine beta-casein promoter region across different bovine breeds
(Cambridge Journals, 2006-02-14)The bovine β-casein (CSN2) gene has been shown to span a region of 8·5 kb, containing nine exons and eight intervening introns (Bonsing et al. 1988; Martin et al. 2002). The exons range in size from 24 to 498 bp; the ... -
Cloning and characterization of two genes encoding rainbow trout homologues of the IFITM protein family
(Elsevier, 2006-04-15)To identify genes connected with leucocyte function in rainbow trout a cDNA library derived front head kidney leucocytes exposed to phytohemagglutinin (PHA) was screened. A clone with homology to the previously described ... -
Truncation error in mesh-free particle methods
(2006-06-25)A truncation error analysis has been developed for the approximation of spatial derivatives in smoothed particle hydrodynamics (SPH) and related first-order consistent methods such as the first-order form of the reproducing ... -
Global gene expression analysis of the effects of Vinblastine on endothelial cells, when eluted from a thermo-responsive polymer
(Wiley Online Library, 2006-06-30)In-stent restenosis remains a significant problem associated with bare metal stents. This drawback has prompted research into improving stent design and the development of novel coatings, including drug-eluting stents. A ... -
Rho A/Rho kinase: human umbilical artery mRNA expression in normal and pre eclamptic pregnancies and functional role in isoprostane-induced vasoconstriction
(Society for Reproduction and Fertility, 2006-07-01)Pre eclampsia represents a state of increased or prolonged vasoconstriction, partially linked to the potent vasocontractile effect of isoprostanes. The process of Rho A-mediated calcium sensitisation is inherent to a state ... -
Engineering assisted surgery: A route for digital design and manufacturing of customized maxillofacial implants
(Elsevier, 2007)Rapid prototyping (RP) and more recently rapid manufacturing (RM) as a part of Engineering Assisted Surgery enables manufacturing of customised implants and prostheses prior to surgical procedures. Beginning with CT ... -
Dependence of mechanical properties of polyamide components on build parameters in the SLS process
(Elsevier, 2007)Customised properties of parts manufactured using the selective laser sintering process are achievable by variation of build parameters. The energy density, controlled by laser power, distance between scan lines and speed ... -
Bovine kappa-casein gene promoter haplotypes with potential implications for milk protein expression
(Elsevier ScienceDirect, 2007-05-25)Genetic analysis of the kappa-casein gene (CSN3) promoter regions of 42 cattle representing 9 different breeds revealed that 2 distinct haplotypes (A and B) exist at this locus, differing from each other by single base ... -
Models of flow-induced loading on blood cells in laminar and turbulent flow, with application to cardiovascular device flow
(Springer, 2007-08)Viscous shear stress and Reynolds stress are often used to predict hemolysis and thrombosis due to flow-induced stress on blood elements in cardiovascular devices. These macroscopic stresses are distinct from the true ... -
Differential expression of the metalloproteinase MMP3 and the a5 integrin subunit in human myometrium at labour
(Oxford University Press, 2007-09-13)Extensive tissue remodelling occurs in the human myometrium before, during and after parturition. The aim of this study was to investigate the expression of two tissue remodelling molecules, matrix metalloproteinase 3 ... -
A note on the evaluation of a beta-casein variant in bovine breeds by allele-specific PCR and relevance to beta-casomorphin
(Teagasc; De Gruyter Open, 2008)Two genetic variants of the bovine beta-casein gene (A(1) and B) encode a histidine residue at codon 67, resulting in potential liberation of a bioactive peptide, beta-casomorphin, upon digestion. An allele-specific PCR ...