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dc.contributor.authorCarleton, Laura A.
dc.contributor.authorChakravarthy, Reka
dc.contributor.authorvan der Sloot, Almer M.
dc.contributor.authorMnich, Katarzyna
dc.contributor.authorSerrano, Luis
dc.contributor.authorSamali, Afshin
dc.contributor.authorGorman, Adrienne M.
dc.date.accessioned2017-11-24T13:48:21Z
dc.date.issued2017-11-03
dc.identifier.citationCarleton, Laura A., Chakravarthy, Reka, van der Sloot, Almer M., Mnich, Katarzyna, Serrano, Luis, Samali, Afshin, & Gorman, Adrienne M. Generation of rationally-designed nerve growth factor (NGF) variants with receptor specificity. Biochemical and Biophysical Research Communications. doi: https://doi.org/10.1016/j.bbrc.2017.11.003en_IE
dc.identifier.issn1090-2104
dc.identifier.urihttp://hdl.handle.net/10379/6980
dc.description.abstractNerve growth factor (NGF) is the prototypic member of the neurotrophin family and binds two receptors, TrkA and the 75 kDa neurotrophin receptor (p75NTR), through which diverse and sometimes opposing effects are mediated. Using the FoldX protein design algorithm, we generated eight NGF variants with different point mutations predicted to have altered binding to TrkA or p75NTR. Of these, the I31R NGF variant exhibited specific binding to p75NTR. The generation of this NGF variant with selective affinity for p75NTR can be used to enhance understanding of neurotrophin receptor imbalance in diseases and identifies a key targetable residue for the development of small molecules to disrupt binding of NGF to TrkA with potential uses in chronic pain.en_IE
dc.description.sponsorshipThis material is based upon works supported by the Science Foundation Ireland under Grant No. 09/RFP/BMT2153, Enterprise Ireland (IP 2016 0480), the Higher Education Authority [PRTLI 5] and the Beckman Fund, School of Natural Sciences, NUI Galway, Ireland.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherElsevieren_IE
dc.relation.ispartofBiochemical And Biophysical Research Communicationsen
dc.subjectFoldXen_IE
dc.subjectNGFen_IE
dc.subjectp75 neurotrophin receptor (p75NTR)en_IE
dc.subjectTrkAen_IE
dc.titleGeneration of rationally-designed nerve growth factor (NGF) variants with receptor specificityen_IE
dc.typeArticleen_IE
dc.date.updated2017-11-21T23:48:26Z
dc.identifier.doi10.1016/j.bbrc.2017.11.003
dc.local.publishedsourcehttps://doi.org/10.1016/j.bbrc.2017.11.003en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|1267881|~|1267883|~|1267869|~|
dc.description.embargo2018-11-03
dc.internal.rssid13480445
dc.local.contactKatarzyna Mnich, Biochemistry, School Of Natural Sciences, Bioresearch Building, Nui. Email: katarzyna.mnich@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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