Show simple item record

dc.contributor.authorCoughlan, Helena
dc.contributor.authorReddington, Kate
dc.contributor.authorTuite, Nina
dc.contributor.authorWee Boo, Teck
dc.contributor.authorCormican, Martin
dc.contributor.authorBarrett, Louise
dc.contributor.authorSmith, Terry
dc.contributor.authorClancy, Eoin
dc.contributor.authorBarry, Thomas
dc.date.accessioned2016-04-18T09:53:50Z
dc.date.issued2015-06-23
dc.identifier.citationHelena Coughlan, Kate Reddington, Nina Tuite, Teck Wee Boo, Martin Cormican, Louise Barrett, Terry J Smith, Eoin Clancy, Thomas Barry (2015) 'Comparative genome analysis identifies novel nucleic acid diagnostic targets for use in the specific detection of Haemophilus influenzae'. Diagnostic Microbiology And Infectious Disease, 83 (2):112-116.en_IE
dc.identifier.issn1879-0070
dc.identifier.urihttp://hdl.handle.net/10379/5684
dc.description.abstractHaemophilus influenzae is recognised as an important human pathogen associated with invasive infections, including bloodstream infection and meningitis. Currently used molecular-based diagnostic assays lack specificity in correctly detecting and identifying H. influenzae. As such, there is a need to develop novel diagnostic assays for the specific identification of H. influenzae. Whole genome comparative analysis was performed to identify putative diagnostic targets, which are unique in nucleotide sequence to H. influenzae. From this analysis, we identified 2 H. influenzae putative diagnostic targets, phoB and pstA, for use in real-time PCR diagnostic assays. Real-time PCR diagnostic assays using these targets were designed and optimised to specifically detect and identify all 55 H. influenzae strains tested. These novel rapid assays can be applied to the specific detection and identification of H. influenzae for use in epidemiological studies and could also enable improved monitoring of invasive disease caused by these bacteria.en_IE
dc.description.sponsorshipScience Foundation Ireland as part of the Biomedical Diagnostics Institute Centre for Science Excellence and Technology (10/CE/B1821)en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherElsevieren_IE
dc.relation.ispartofDiagnostic Microbiology And Infectious Diseaseen
dc.subjectH. influenzaeen_IE
dc.subjectGenome comparisonen_IE
dc.subjectReal-time PCRen_IE
dc.subjectphoBen_IE
dc.subjectpstAen_IE
dc.subjectMicrobiologyen_IE
dc.titleComparative genome analysis identifies novel nucleic acid diagnostic targets for use in the specific detection of Haemophilus influenzaeen_IE
dc.typeArticleen_IE
dc.date.updated2016-04-18T09:29:20Z
dc.identifier.doi10.1016/j.diagmicrobio.2015.06.013
dc.local.publishedsourcehttp://www.sciencedirect.com/science/article/pii/S0732889315002163en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|1267880|~|
dc.description.embargo2016-06-23
dc.internal.rssid10295821
dc.local.contactThomas Barry, Dept. Of Microbiology, Arts/Science Building, Nui Galway. 3189 Email: thomas.barry@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
nui.item.downloads391


Files in this item

Attribution-NonCommercial-NoDerivs 3.0 Ireland
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. Please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.

The following license files are associated with this item:

Thumbnail

This item appears in the following Collection(s)

Show simple item record