dc.contributor.author | Reddington, Kate | |
dc.contributor.author | Schwenk, Stefan | |
dc.contributor.author | Tuite, Nina | |
dc.contributor.author | Platt, Gareth | |
dc.contributor.author | Davar, Danesh | |
dc.contributor.author | Coughlan, Helena | |
dc.contributor.author | Personne, Yoann | |
dc.contributor.author | Enne, Virve I. | |
dc.contributor.author | Zumla, Alimuddin | |
dc.contributor.author | Barry, Thomas | |
dc.date.accessioned | 2016-04-11T08:34:47Z | |
dc.date.available | 2016-04-11T08:34:47Z | |
dc.date.issued | 2015-06-23 | |
dc.identifier.citation | Kate Reddington, Stefan Schwenk, Nina Tuite, Gareth Platt, Danesh Davar, Helena Coughlan, Yoann Personne, Vanya Gant, Virve I. Enne, Alimuddin Zumla, and Thomas Barry (2015) 'Comparison of established diagnostic methodologies and a novel bacterial smpB real-time PCR assay for the specific detection of Haemophilus influenzae associated with respiratory tract infections'. Journal Of Clinical Microbiology, . | en_IE |
dc.identifier.issn | 1098-660X | |
dc.identifier.uri | http://hdl.handle.net/10379/5651 | |
dc.description.abstract | Haemophilus influenzae is a significant causative agent of respiratory tract infections (RTI) worldwide. The development of a
rapid H. influenzae diagnostic assay that would allow for the implementation of infection control measures and also improve
antimicrobial stewardship for patients is required. A number of nucleic acid diagnostics approaches that detect H. influenzae in
RTIs have been described in the literature; however, there are reported specificity and sensitivity limitations for these assays. In
this study, a novel real-time PCR diagnostic assay targeting the smpB gene was designed to detect all serogroups of H. influenzae.
The assay was validated using a panel of well-characterized Haemophilus spp. Subsequently, 44 Haemophilus clinical isolates
were collected, and 36 isolates were identified as H. influenzae using a gold standard methodology that combined the results of
matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) and a fucK diagnostic assay.
Using the novel smpB diagnostic assay, 100% concordance was observed with the gold standard, demonstrating a sensitivity of
100% (95% confidence interval [CI], 90.26% to 100.00%) and a specificity of 100% (95% CI, 63.06% to 100.00%) when used on
clinical isolates. To demonstrate the clinical utility of the diagnostic assay presented, a panel of lower RTI samples (n 98) were
blindly tested with the gold standard and smpB diagnostic assays. The results generated were concordant for 94/98 samples
tested, demonstrating a sensitivity of 90.91% (95% CI, 78.33% to 97.47%) and a specificity of 100% (95% CI, 93.40% to 100.00%)
for the novel smpB assay when used directly on respiratory specimens. | en_IE |
dc.description.sponsorship | All authors with the exception of H.C. are supported by EC Grant RiDRTI
FP7-Health-2012-INNOVATION-2 (grant agreement 304865). | en_IE |
dc.format | application/pdf | en_IE |
dc.language.iso | en | en_IE |
dc.publisher | American Society for Microbiology | en_IE |
dc.relation.ispartof | Journal Of Clinical Microbiology | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | Haemophilus influenzae | en_IE |
dc.subject | Respiratory tract infections | en_IE |
dc.subject | Diagnostic methodologies | en_IE |
dc.subject | Bacterial smpB real-time PCR assay | en_IE |
dc.subject | Microbiology | en_IE |
dc.title | Comparison of established diagnostic methodologies and a novel bacterial smpB real-time PCR assay for the specific detection of haemophilus influenzae associated with respiratory tract infections | en_IE |
dc.type | Article | en_IE |
dc.date.updated | 2016-04-06T13:27:16Z | |
dc.identifier.doi | 10.1128/JCM.00777-15 | |
dc.local.publishedsource | http://jcm.asm.org/content/53/9/2854.full.pdf+html | en_IE |
dc.description.peer-reviewed | peer-reviewed | |
dc.contributor.funder | |~|1267872|~| | |
dc.internal.rssid | 9020252 | |
dc.local.contact | Thomas Barry, Dept. Of Microbiology, Arts/Science Building, Nui Galway. 3189 Email: thomas.barry@nuigalway.ie | |
dc.local.copyrightchecked | No | |
dc.local.version | ACCEPTED | |
nui.item.downloads | 286 | |