Browsing Biochemistry (Scholarly Articles) by Author "Ramadurai, Sivaramakrishnan"
Now showing items 1-2 of 2
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Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy
Broderick, Ronan; Ramadurai, Sivaramakrishnan; Tóth, Katalin; Togashi, Denisio M.; Ryder, Alan G.; Langowski, Jörg; Nasheuer, Heinz-Peter (Public Library of Science, 2012-04-19)Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous ... -
Optimizing fluorescent protein expression for quantitative fluorescence microscopy and spectroscopy using herpes simplex thymidine kinase promoter sequences
Ali, Rizwan; Ramadurai, Sivaramakrishnan; Barry, Frank; Nasheuer, Heinz-Peter (Wiley, 2018-04-16)The modulation of expression levels of fluorescent fusion proteins (FFPs) is central for recombinant DNA technologies in modern biology as overexpression of proteins contributes to artifacts in biological experiments. In ...