Browsing Regenerative Medicine Institute (Scholarly Articles) by Issue Date
Now showing items 1-20 of 39
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Gene therapy in transplantation: Toward clinical trials
(Thompson, 2009)The genetic modification of organs or cells is an attractive approach to protect allogeneic transplants from acute rejection and other complications. The transplant setting offers a unique opportunity to utilise ex vivo ... -
Genetically Modified Mesenchymal Stem
(2010-03)Adult mesenchymal stem cells (MSCs) are non-hematopoietic cells with multi-lineage potential to differentiate into various tissues of mesodermal origin. They can be isolated from bone marrow and other tissues and have the ... -
A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model
(PubMed Central, 2011)Purpose: To establish a cornea transplant model in a pigmented rat strain and to define the immunologic reaction toward corneal allografts, by studying the cellular and humoral immune response after keratoplasty. Methods:Full ... -
Adenoviral transduction of mesenchymal stem cells: in vitro responses and in vivo immune responses after cell transplantation
(2012)Adult mesenchymal stem cells (MSCs) are non-hematopoietic cells with multi-lineage potential which makes them attractive targets for regenerative medicine applications. However, to date, therapeutic success of MSC-therapy ... -
Immunogenicity of allogeneic mesenchymal stem cells
(2012)Mesenchymal stem cells (MSCs) inhibit proliferation of allogeneic T cells and express low levels of major histocompatibility complex class I (MHCI), MHCII and vascular adhesion molecule-1 (VCAM-1). We investigated whether ... -
Can a young muscle s stem cell secretome prolong our lives?
(2012)Ageing is a biological certainty for all living organisms, and is due to the loss of tissue homeostasis and regenerative capacity (except for newts) in which somatic stem cells are thought to play an important role. ... -
Donor bone marrow-derived dendritic cells prolong corneal allograft survival and promote an intragraft immunoregulatory milieu.
(Nature Publishing Group, 2013-08)Investigations into cell therapies for application in organ transplantation have grown. Here, we describe the ex vivo generation of donor bone marrow-derived dendritic cells (BMDCs) and glucocorticoid-treated BMDCs with ... -
Effects of growth phase and temperature on sigma(B) Activity within a Listeria monocytogenes population: evidence for RsbV-independent activation of sigma(B) at refrigeration temperatures
(Hindawi Publishing Corporation, 2014-03-05)The alternative sigma factor sigma(B) of Listeria monocytogenes is responsible for regulating the transcription of many of the genes necessary for adaptation to both food-related stresses and to conditions found within the ... -
Evaluation of cartilage repair by mesenchymal stem cells seeded on a PEOT/PBT scaffold in an osteochondral defect
(Springer Verlag, 2015-01-15)The main objective of this study was to evaluate the effectiveness of a mesenchymal stem cell (MSC)-seeded polyethylene-oxide-terephthalate/polybutylene-terephthalate (PEOT/PBT) scaffold for cartilage tissue repair in an ... -
Inefficiency in macromolecular transport of SCS‐based microcapsules affects viability of primary human mesenchymal stem cells but not of immortalized cells
(Wiley, 2015-05-01)Microcapsules made of sodium cellulose sulphate (SCS) and poly-diallyl-dimethyl-ammonium chloride (pDADMAC) have been employed to encapsulate a wide range of established cell lines for several applications. However, little ... -
Evaluation of the early in vivo response of a functionally graded macroporous scaffold in an osteochondral defect in a rabbit model
(Springer Verlag, 2015-10-05)Cartilage tissue engineering is a multifactorial problem requiring a wide range of material property requirements from provision of biological cues to facilitation of mechanical support in load-bearing diarthrodial joints. ... -
Endothelial nitric oxide synthase induces heat shock protein HSPA6 (HSP70B ) in human arterial smooth muscle cells
(Elsevier, 2015-12-01)Endothelial nitric oxide synthase (eNOS) is the major source of nitric oxide (NO) production in blood vessels. One of the pleitropic functions of eNOS derived NO is to inhibit vascular smooth muscle cell proliferation ... -
Differentiation of vascular stem cells contributes to ectopic calcification of atherosclerotic plaque
(Wiley, 2016-02-27)The cellular and molecular basis of vascular calcification (VC) in atherosclerosis is not fully understood. Here, we investigate role of resident/circulating progenitor cells in VC and contribution of inflammatory plaque ... -
Sterile post-traumatic immunosuppression
(Nature Publishing Group, 2016-04-29)After major trauma, the human immune system initiates a series of inflammatory events at the injury site that is later followed by suppression of local inflammation favoring the repair and remodeling of the damaged tissues. ... -
In vitro models for assessing therapeutic angiogenesis
(Elsevier, 2016-06-01)Arterial obstruction leading to ischemia causes a reduction of oxygen and nutrient supply to distal tissues. The physiological response to tissue ischemia triggers a cascade of events that results in the development of ... -
Development of a flow cytometry-based potency assay for measuring the in vitro immunomodulatory properties of mesenchymal stromal cells
(Elsevier, 2016-07-20)Human bone marrow-derived mesenchymal stromal/stem cells (MSC) have well-documented modulatory effects on multiple immune cell types. Although these effects are linked to their therapeutic benefit in diverse diseases, a ... -
Permissive roles of cytokines Interleukin-7 and Flt3-ligand in mouse B cell lineage commitment
(National Academy of Sciences, 2016-11-04)Hematopoietic cells are continuously generated throughout life from hematopoietic stem cells, thus making hematopoiesis a favorable system to study developmental cell lineage commitment. The main factors incorporating ... -
Pro-B cells propagated in stromal cell-free cultures reconstitute functional B-cell compartments in immunodeficient mice
(Wiley, 2016-12-28)Up to now long-term in vitro growth of pro-B cells was thought to require stromal cells. However, here we show that fetal liver (FL) and bone marrow (BM) derived pro-B cells can be propagated long-term in stromal cell-free ... -
Biodistribution and retention of locally administered human mesenchymal stromal cells: Quantitative polymerase chain reaction–based detection of human DNA in murine organs
(Elsevier, 2017-01-12)Background. Determining the distributive fate and retention of a cell therapy product after administration is an essential part of characterizing it's biosafety profile. Therefore, regulatory guidelines stipulate that ... -
Anti-donor antibody induction following intramuscular injections of allogeneic mesenchymal stromal cells
(Wiley, 2018-02-15)Allogeneic mesenchymal stromal cells (allo-MSC) are a promising "off-the-shelf" therapy with anti-inflammatory and pro-repair properties. This study investigated humoral immune responses to intramuscular (IM) injections ...