Evaluation of cartilage repair by mesenchymal stem cells seeded on a PEOT/PBT scaffold in an osteochondral defect
Van Blitterswijk, Clemen
MetadataShow full item record
This item's downloads: 155 (view details)
Barron, V,Merghani, K,Shaw, G,Coleman, CM,Hayes, JS,Ansboro, S,Manian, A,O'Malley, G,Connolly, E,Nandakumar, A,van Blitterswijk, CA,Habibovic, P,Moroni, L,Shannon, F,Murphy, JM,Barry, F (2015) 'Evaluation of Cartilage Repair by Mesenchymal Stem Cells Seeded on a PEOT/PBT Scaffold in an Osteochondral Defect'. Annals Of Biomedical Engineering, 43 :2069-2082.
The main objective of this study was to evaluate the effectiveness of a mesenchymal stem cell (MSC)-seeded polyethylene-oxide-terephthalate/polybutylene-terephthalate (PEOT/PBT) scaffold for cartilage tissue repair in an osteochondral defect using a rabbit model. Material characterisation using scanning electron microscopy indicated that the scaffold had a 3D architecture characteristic of the additive manufacturing fabrication method, with a strut diameter of 296 +/- A 52 mu m and a pore size of 512 +/- A 22 mu m x 476 +/- A 25 mu m x 180 +/- A 30 mu m. In vitro optimisation revealed that the scaffold did not generate an adverse cell response, optimal cell loading conditions were achieved using 50 mu g/ml fibronectin and a cell seeding density of 25 x 10(6) cells/ml and glycosaminoglycan (GAG) accumulation after 28 days culture in the presence of TGF beta 3 indicated positive chondrogenesis. Cell-seeded scaffolds were implanted in osteochondral defects for 12 weeks, with cell-free scaffolds and empty defects employed as controls. On examination of toluidine blue staining for chondrogenesis and GAG accumulation, both the empty defect and the cell-seeded scaffold appeared to promote repair. However, the empty defect and the cell-free scaffold stained positive for collagen type I or fibrocartilage, while the cell-seeded scaffold stained positive for collagen type II indicative of hyaline cartilage and was statistically better than the cell-free scaffold in the blinded histological evaluation. In summary, MSCs in combination with a 3D PEOT/PBT scaffold created a reparative environment for cartilage repair.
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. Please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.
The following license files are associated with this item: