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dc.contributor.authorTikhanovich, Irina
dc.contributor.authorLiang, Bo
dc.contributor.authorSeoighe, Cathal
dc.contributor.authorFolk, William R.
dc.contributor.authorNasheuer, Heinz-Peter
dc.date.accessioned2021-01-07T10:10:04Z
dc.date.available2021-01-07T10:10:04Z
dc.date.issued2011
dc.identifier.citationTikhanovich, Irina, Liang, Bo, Seoighe, Cathal, Folk, William R., & Nasheuer, Heinz Peter. (2011). Inhibition of Human BK Polyomavirus Replication by Small Noncoding RNAs. Journal of Virology, 85(14), 6930. doi:10.1128/JVI.00547-11en_IE
dc.identifier.issn0022-538X
dc.identifier.urihttp://hdl.handle.net/10379/16423
dc.description.abstractSmall noncoding RNAs regulate a variety of cellular processes, including genomic imprinting, chromatin remodeling, replication, transcription, and translation. Here, we report small replication-regulating RNAs (srRNAs) that specifically inhibit DNA replication of the human BK polyomavirus (BKV) in vitro and in vivo. srRNAs from FM3A murine mammary tumor cells were enriched by DNA replication assay-guided fractionation and hybridization to the BKV noncoding control region (NCCR) and synthesized as cDNAs. Selective mutagenesis of the cDNA sequences and their putative targets suggests that the inhibition of BKV DNA replication is mediated by srRNAs binding to the viral NCCR, hindering early steps in the initiation of DNA replication. Ectopic expression of srRNAs in human cells inhibited BKV DNA replication in vivo. Additional srRNAs were designed and synthesized that specifically inhibit simian virus 40 (SV40) DNA replication in vitro. These observations point to novel mechanisms for regulating DNA replication and suggest the design of synthetic agents for inhibiting replication of polyomaviruses and possibly other viruses.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherAmerican Society for Microbiology Journalsen_IE
dc.relation.ispartofVirology Journalen
dc.subjectDNA-POLYMERASE-ALPHAen_IE
dc.subjectSPECIES-SPECIFIC REPLICATIONen_IE
dc.subjectIN-VITRO REQUIRESen_IE
dc.subjectLARGE T-ANTIGENen_IE
dc.subjectSIMIAN VIRUS-40en_IE
dc.subjectSV40 ORIGINen_IE
dc.subjectTHERMODYNAMIC PARAMETERSen_IE
dc.subjectPREDICT STABILITYen_IE
dc.subjectCHROMOSOMAL DNAen_IE
dc.subjectTOPOISOMERASE-Ien_IE
dc.titleInhibition of human BK polyomavirus replication by small noncoding RNAsen_IE
dc.typeArticleen_IE
dc.date.updated2021-01-05T21:41:09Z
dc.identifier.doi10.1128/JVI.00547-11
dc.local.publishedsourcehttps://doi.org/10.1128/JVI.00547-11en_IE
dc.description.peer-reviewedpeer-reviewed
dc.internal.rssid16223965
dc.local.contactHeinz-Peter Nasheuer, 2fn, Rm Bms 2010, Biochemistry S, Bioresearch Building, Nui Galway, Galway. 2430 Email: heinz.nasheuer@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionPUBLISHED
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