dc.contributor.author | Tsiapalis, Dimitrios | |
dc.contributor.author | De Pieri, Andrea | |
dc.contributor.author | Spanoudes, Kyriakos | |
dc.contributor.author | Sallent, Ignacio | |
dc.contributor.author | Kearns, Stephen | |
dc.contributor.author | Kelly, Jack L. | |
dc.contributor.author | Raghunath, Michael | |
dc.contributor.author | Zeugolis, Dimitrios I. | |
dc.date.accessioned | 2020-04-22T10:43:08Z | |
dc.date.issued | 2020-02-26 | |
dc.identifier.citation | Tsiapalis, Dimitrios, De Pieri, Andrea, Spanoudes, Kyriakos, Sallent, Ignacio, Kearns, Stephen, Kelly, Jack L., Raghunath, Michael, Zeugolis, Dimitrios I. (2020). The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents. Biofabrication, 12(2), 025018. doi:10.1088/1758-5090/ab6412 | en_IE |
dc.identifier.issn | 1758-5090 | |
dc.identifier.uri | http://hdl.handle.net/10379/15902 | |
dc.description.abstract | Cellular therapies play an important role in tendon tissue engineering, with tenocytes being the most prominent and potent cell population available. However, for the development of a rich extracellular matrix tenocyte-assembled tendon equivalent, prolonged in vitro culture is required, which is associated with phenotypic drift. Recapitulation of tendon tissue microenvironment in vitro with cues that enhance and accelerate extracellular matrix synthesis and deposition, whilst maintaining tenocyte phenotype, may lead to functional cell therapies. Herein, we assessed the synergistic effect of low oxygen tension (enhances extracellular matrix synthesis) and macromolecular crowding (enhances extracellular matrix deposition) in human tenocyte culture. Protein analysis demonstrated that human tenocytes at 2% oxygen tension and with 50 μg ml−1 carrageenan (macromolecular crowder used) significantly increased synthesis and deposition of collagen types I, III, V and VI. Gene analysis at day 7 illustrated that human tenocytes at 2% oxygen tension and with 50 μg ml−1 carrageenan significantly increased the expression of prolyl 4-hydroxylase subunit alpha 1, procollagen-lysine 2- oxoglutarate 5-dioxygenase 2, scleraxis, tenomodulin and elastin, whilst chondrogenic (e.g. runt-related transcription factor 2, cartilage oligomeric matrix protein, aggrecan) and osteogenic (e.g. secreted phosphoprotein 1, bone gamma-carboxyglutamate protein) trans-differentiation markers were significantly down-regulated or remained unchanged. Collectively, our data clearly illustrates the beneficial synergistic effect of low oxygen tension and macromolecular crowding in the accelerated development of tissue equivalents. | en_IE |
dc.description.sponsorship | This work was supported by: H2020, Marie Skłodowska-Curie Actions, Innovative Training Networks 2015 Tendon Therapy Train project (Grant No. 676338); H2020, Wide Spread Coordination and Support Action, twinning 2017 Achilles project (Grant No. 810850); Science Foundation Ireland, Career Development Award (Grant No. 15/CDA/3629); and Science Foundation Ireland / European Regional Development Fund (Grant No. 13/RC/2073). | en_IE |
dc.format | application/pdf | en_IE |
dc.language.iso | en | en_IE |
dc.publisher | IOP Publishing | en_IE |
dc.relation.ispartof | Biofabrication | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | Cell therapies | en_IE |
dc.subject | Tendon tissue engineering | en_IE |
dc.subject | Tenocyte phenotype | en_IE |
dc.subject | In vitro microenvironment | en_IE |
dc.subject | Excluding volume effect | en_IE |
dc.subject | Hypoxia | en_IE |
dc.subject | Normoxia | en_IE |
dc.title | The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents | en_IE |
dc.type | Article | en_IE |
dc.date.updated | 2020-04-21T08:37:20Z | |
dc.identifier.doi | 10.1088/1758-5090/ab6412 | |
dc.local.publishedsource | https://doi.org/10.1088/1758-5090/ab6412 | en_IE |
dc.description.peer-reviewed | peer-reviewed | |
dc.contributor.funder | Horizon 2020 | en_IE |
dc.contributor.funder | Science Foundation Ireland | en_IE |
dc.contributor.funder | European Regional Development Fund | en_IE |
dc.description.embargo | 2021-02-26 | |
dc.internal.rssid | 20707219 | |
dc.local.contact | Dimitros Tsiapalis, Remodel, Centre For Research In Medical Devices , Biomedical Sciences Building, Nui Galway. Email: d.tsiapalis2@nuigalway.ie | |
dc.local.copyrightchecked | Yes | |
dc.local.version | ACCEPTED | |
dcterms.project | info:eu-repo/grantAgreement/EC/H2020::MSCA-ITN-ETN/676338/EU/Engineering in vitro microenvironments for translation of cell-based therapies for tendon repair/Tendon Therapy Train | en_IE |
dcterms.project | info:eu-repo/grantAgreement/EC/H2020::CSA/810850/EU/Overcoming specific weaknesses in tendon biology to design advanced regenerative therapies/Achilles | en_IE |
dcterms.project | info:eu-repo/grantAgreement/SFI/SFI Career Development Award/15/CDA/3629/IE/Tissue Engineered Nanoassemblies _ Advanced Biomimicry of Living Equivalents (Short Title: TENABLE)/ | en_IE |
dcterms.project | info:eu-repo/grantAgreement/SFI/SFI Research Centres/13/RC/2073/IE/C�RAM - Centre for Research in Medical Devices/ | en_IE |
nui.item.downloads | 84 | |