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dc.contributor.authorLi, Boyan
dc.contributor.authorRyan, Paul W.
dc.contributor.authorRay, Bryan H.
dc.contributor.authorSirimuthu, Narayana M. S.
dc.contributor.authorRyder, Alan G.
dc.date.accessioned2012-08-23T11:20:15Z
dc.date.available2012-08-23T11:20:15Z
dc.date.issued2010
dc.identifier.citationLi B., Ryan P.W., Ray B.H., Leister K.J., Sirimuthu N.M.S., Ryder A.G. (2010) 'Rapid characterization and quality control of complex cell culture media solutions using Raman spectroscopy and chemometrics'. Biotechnology And Bioengineering, 107 (2):290-301.en_US
dc.identifier.issn1097-0290
dc.identifier.urihttp://hdl.handle.net/10379/2942
dc.descriptionJournal articleen_US
dc.description.abstractThe use of Raman spectroscopy coupled with chemometrics for the rapid identification, characterization, and quality assessment of Complex cell culture media components used for industrial mammalian cell culture was investigated. Raman spectroscopy offers significant advantages for the analysis of complex, aqueous-based materials used in biotechnology because there is no need for sample preparation and water is a weak Raman scatterer. We demonstrate the efficacy of the method for the routine analysis of dilute aqueous solution of five different chemically defined (CD) commercial media components used in a Chinese Hamster Ovary (CHO) cell manufacturing process for recombinant proteins. The chemometric processing of the Raman spectral data is the key factor in developing robust methods. Here, we discuss the optimum methods for eliminating baseline drift, background fluctuations, and other instrumentation artifacts to generate reproducible spectral data. Principal component analysis (PCA) and soft independent modeling of class analogy (SIMCA) were then employed in the development of a robust routine for both identification and quality evaluation of the five different media components. These methods have the potential to be extremely useful in an industrial context for "in-house"; sample handling, tracking, and quality control. Biotechnol. Bioeng. 2010;107: 290-301. (c) 2010 Wiley Periodicals, Inc.en_US
dc.description.sponsorshipIndustrial Development Authority; Bristol¿Myers Squibb [Centre for Bioanalytical Sciences]en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.relation.ispartofBiotechnology And Bioengineeringen
dc.subjectRaman spectroscopyen_US
dc.subjectChemometricsen_US
dc.subjectCell cultureen_US
dc.subjectChemically defined mediaen_US
dc.subjectPCAen_US
dc.subjectSIMCAen_US
dc.subjectPrincipal component analysisen_US
dc.subjectNear-infrared spectroscopyen_US
dc.subjectSolid mixturesen_US
dc.subjectBiomedical applicationsen_US
dc.subjectBiopsy specimensen_US
dc.subjectCereal foodsen_US
dc.subjectSerum-freeen_US
dc.subjectClassificationen_US
dc.titleRapid characterization and quality control of complex cell culture media solutions using Raman spectroscopy and chemometricsen_US
dc.typeArticleen_US
dc.date.updated2012-08-17T14:05:38Z
dc.identifier.doiDOI 10.1002/bit.22813
dc.local.publishedsourcehttp://dx.doi.org/10.1002/bit.22813en_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|Other|~|
dc.internal.rssid1159083
dc.local.contactAlan Ryder, School Of Chemistry, Room 228, Arts/Science Building, Nui Galway. 2943 Email: alan.ryder@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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