Browsing National Centre for Biomedical Engineering Science by Author "|~|"
Now showing items 21-40 of 42
-
Functional coupling of beta(3)-adrenoceptors and large conductance calcium-activated potassium channels in human uterine myocytes
Doheny, Helen C.; Lynch, Caoimhe M.; Smith, Terry; Morrison, John J. (Endocrine Society, 2005-07-02)Context: beta(3)-Adrenoreceptor modulation in human myometrium during pregnancy is linked functionally to myometrial inhibition. Maxi-K+ channels (BKCa) play a significant role in modulating cell membrane potential and ... -
Ghrelin in the human myometrium
Smith, Terry; O'Brien, Margaret; Morrison, John J. (2010)Background: Ghrelin is a 28-amino acid octanolyated peptide, synthesised primarily in the stomach. It stimulates growth hormone release, food intake and exhibits many other diverse effects. Our group have previously ... -
Global gene expression analysis of the effects of Vinblastine on endothelial cells, when eluted from a thermo-responsive polymer
McLucas, Emily; Rochev, Yury; Carroll, W. M.; Smith, Terry (Wiley Online Library, 2006-06-30)In-stent restenosis remains a significant problem associated with bare metal stents. This drawback has prompted research into improving stent design and the development of novel coatings, including drug-eluting stents. A ... -
Human chorionic gonadotrophin relaxation of human pregnant myometrium and activation of the BKCa channel
Doheny, HC,Houlihan, DD,Ravikumar, N,Smith, TJ,Morrison, JJ (2003)The uterorelaxant effect of human chorionic gonadotropin (hCG) is regarded as an important mediator in maintenance of uterine quiescence during pregnancy with clinical potential for tocolysis, the mechanisms of which are ... -
Interaction of cell culture with composition effects on the mechanical properties of polycaprolactone-hydroxyapatite scaffolds fabricated via selective laser sintering (SLS)
Eosoly, Szilvia; Vrana, Nihal Engin; Lohfeld, Stefan; Hindie, Mathilde; Looney, Lisa (Elsevier, 2012)In the current study PCL/HA composites were fabricated using SLS as two- and three-dimensional lattice structures and exposed to a cellular component (MC 313 osteoblast-like cells). The main aims were to determine the ... -
Isolation and characterization of the bovine Stearoyl-CoAdesaturase promoter and analysis of polymorphisms in the promoter region in dairy cows
Keating, Aileen F.; Murphy, John J.; Smith, Terry J.; Cairns, Michael T. (Springer, 2005-03)Conjugated linoleic acid (CLA) in milk arises through microbial biohydrogenation of dietary polyunsaturated fatty acids (PUFA) in the rumen, and by the action of mammary Stearoyl-CoA desaturase (Scd). A large variation ... -
Laser sintering for the fabrication of tissue engineering scaffolds
Lohfeld, Stefan; McHugh, Peter E. (Springer Science+Business Media, 2012)Laser sintering (LS) utilises a laser to sinter powder particles. A volumetric model is sliced and processedcross section by cross section to create a physical part. In theory, all powdered materials are suitable forsintering; ... -
Mesenchymal stem cells and osteoarthritis: remedy or accomplice?
Coleman, Cynthia M.; Curtin, Caroline; Barry, Frank; O'Flatharta, Cathal; Murphy, Mary (Mary Ann Liebert, Inc., 2010-10)Multipotent mesenchymal stromal or stem cells (MSCs) are likely to be agents of connective tissue homeostasis and repair. Because the hallmark of osteoarthritis (OA) is degeneration and failure to repair connective tissues ... -
miRNA signature of unfolded protein response in H9c2 rat cardiomyoblasts
Samali, Afshin (2014)Background: Glucose and oxygen deprivation during ischemia is known to affect the homeostasis of the endoplasmic reticulum (ER) in ways predicted to activate the unfolded protein response (UPR). Activation of UPR signalling ... -
Modulation of human uterine smooth muscle cell collagen contractility by thrombin, Y-27632, TNF alpha and indomethacin
Fitzgibbon, Joan; Morrison, John J.; Smith, Terry; O'Brien, Margaret (BioMed Central, 2009-01-08)Background: Preterm labour occurs in approximately 10% of pregnancies and is a major cause of infant morbidity and mortality. However, the pathways involved in regulating contractility in normal and preterm labour are not ... -
Neuronal glycosylation differentials in normal, injured and chondroitinase-treated environments
Kilcoyne, Michelle; Sharma, Shashank; McDevitt, Niamh; O'Leary, Claire; Joshi, Lokesh; McMahon, Siobhán S. (2012)Glycosylation is found ubiquitously throughout the central nervous system (CNS). Chondroitin sulphate proteoglycans (CSPGs) are a group of molecules heavily substituted with glycosaminoglycans (GAGs) and are found in the ... -
A note on the evaluation of a beta-casein variant in bovine breeds by allele-specific PCR and relevance to beta-casomorphin
Keating, Aileen F.; Smith, Terry J.; Cairns, Michael T. (Teagasc; De Gruyter Open, 2008)Two genetic variants of the bovine beta-casein gene (A(1) and B) encode a histidine residue at codon 67, resulting in potential liberation of a bioactive peptide, beta-casomorphin, upon digestion. An allele-specific PCR ... -
Oxytocic plant cyclotides as templates for peptide G protein-coupled receptor ligand design
O'Brien, Margaret; Smith, Terry (National Academy Of Sciences Of The United States Of America, 2013-10-23)Cyclotides are plant peptides comprising a circular backbone and three conserved disulfide bonds that confer them with exceptional stability. They were originally discovered in Oldenlandia affinis based on their use in ... -
Periodic acid-Schiff's reagent assay for carbohydrates in a microtiter plate format
Kilcoyle, Michelle; Gerlach, Jared Q.; Farrell, Mark P.; Joshi, Lokesh (2011)Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low neutral sugar content. We have adapted and optimised ... -
Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint
Maffini, Stefano; Rainey, Michael D.; Kaczmarczyk, Agnieszka; O'Connor, Aisling; Gaboriau, David; Musacchio, Andrea; Santocanale, Corrado (Company of Biologists, 2016)During mitotic arrest induced by microtubule targeting drugs, the weakening of the spindle assembly checkpoint (SAC) allows cells to progress through the cell cycle without chromosome segregation occurring. PLK1 kinase ... -
Resistance to TRAIL in non-transformed cells is due to multiple redundant pathways
Samali, Afshin (2013)Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) is a cytokine and a selective inducer of apoptosis in a range of tumour cells, but not in normal, untransformed cells. A large number of chemotherapeutics ... -
Rho A/Rho kinase: human umbilical artery mRNA expression in normal and pre eclamptic pregnancies and functional role in isoprostane-induced vasoconstriction
Friel, Anne M,; Sexton, Donal J.; O'Reilly, Michael W.; Smith, Terry; Morrison, John J. (Society for Reproduction and Fertility, 2006-07-01)Pre eclampsia represents a state of increased or prolonged vasoconstriction, partially linked to the potent vasocontractile effect of isoprostanes. The process of Rho A-mediated calcium sensitisation is inherent to a state ... -
A single nucleotide polymorphism in the bovine beta-casein promoter region across different bovine breeds
Keating, Aileen F.; Smith, Terry J.; Cairns, Michael T. (Cambridge Journals, 2006-02-14)The bovine β-casein (CSN2) gene has been shown to span a region of 8·5 kb, containing nine exons and eight intervening introns (Bonsing et al. 1988; Martin et al. 2002). The exons range in size from 24 to 498 bp; the ... -
Surface chemistry and linker effects on lectin-carbohydrate recognition for glycan microarrays
Kilcoyne, Michelle; Gerlach, Jared Q.; Kane, Marian; Joshi, Lokesh (2012)Glycan microarrays are an increasingly utilised tool for analysis of protein-carbohydrate interactions and a variety of glycan-containing molecules and slide chemistries have been used to array carbohydrates on microarray ... -
Testes and brain gene expression in precocious male and adult maturing Atlantic salmon (Salmo salar)
Guiry, Aoife; Flynn, Denis; Hubert, Sophie; O'Keeffe, Allan M; LeProvost, Olivier; White, Samantha L; Forde, Patrick F; Davoren, Pamela; Houeix, Benoit; Smith, Terry; Wilkins, Noel P.; Cairns, Michael T. (BioMed Central, 2010)Background: The male Atlantic salmon generally matures in fresh water upon returning after one or several years at sea. Some fast-growing male parr develop an alternative life strategy where they sexually mature before ...