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Restriction of human polyomavirus BK virus DNA replication in murine cells and extracts
(2009)
BK virus (BKV) causes persistent and asymptomatic infections in most humans and is the etiologic agent of polyomavirus-associated nephropathy (PVAN) and other pathologies. Unfortunately, there are no animal models with ...
SV40 T antigen interactions with ssDNA and replication protein A: a regulatory role of T antigen monomers in lagging strand DNA replication
(Oxford University Press (OUP), 2020-03-04)
DNA replication is a central process in all living organisms. Polyomavirus DNA replication serves as a model system for eukaryotic DNA replication and has considerably contributed to our understanding of basic replication ...
Alkoxyalkyl esters of nucleotide analogs inhibit polyomavirus DNA replication and large T antigen activities
(American Society for Microbiology Journals, 2020-12-07)
Polyomavirus-related infections are ubiqutious in immunocompromised individuals and in some cases are intractable and fatal. Due to lack of approved drugs to treat polyomavirus infections, cidofovir, a phosphonate nucleotide ...
Determination of the number of RAD51 molecules in different human cell lines
(Taylor & Francis, 2019-11-15)
Knowledge about precise numbers of specific molecules is necessary for understanding and verification of biological pathways. The RAD51 protein is central in the repair of DNA double-strand breaks (DSBs) by homologous ...
ATPase activity of human binding immunoglobulin protein (BiP) variants is enhanced by signal sequence and physiological concentrations of Mn2+
(Wiley Open Access, 2019-08-29)
B-cell immunoglobulin binding protein (BiP) is an essential endoplasmic reticulum (ER) chaperone normally found in the ER lumen. However, BiP also has other extracellular and intracellular functions. As it is unclear whether ...
Inhibition of human BK polyomavirus replication by small noncoding RNAs
(American Society for Microbiology Journals, 2011)
Small noncoding RNAs regulate a variety of cellular processes, including genomic imprinting, chromatin remodeling, replication, transcription, and translation. Here, we report small replication-regulating RNAs (srRNAs) ...
Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy
(Public Library of Science, 2012-04-19)
Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous ...
Cell cycle-dependent formation of Cdc45-Claspin complexes in human cells is compromized by UV-mediated DNA damage
(Wiley, 2013-08-02)
The replication factor Cdc45 has essential functions in the initiation and elongation steps of eukaryotic DNA replication and plays an important role in the intra-S-phase checkpoint. Its interactions with other replication ...
The awarding of the first honorary Doctor of Science by the Queen's University in Ireland to William King – a journey of scientific curiosity
(Royal Irish Academy, 2020)
William King (1809 86) was the founding Professor of Mineralogy and Geology at Queen s College Galway (QCG), one of three regional colleges opened in 1849 to provide secular university-level education in Ireland. King came ...
Optimizing fluorescent protein expression for quantitative fluorescence microscopy and spectroscopy using herpes simplex thymidine kinase promoter sequences
(Wiley, 2018-04-16)
The modulation of expression levels of fluorescent fusion proteins (FFPs) is central for recombinant DNA technologies in modern biology as overexpression of proteins contributes to artifacts in biological experiments. In ...