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dc.contributor.authorRitter, Thomas
dc.identifier.citationSchu, S., Nosov, M., O'Flynn, L., Shaw, G., Treacy, O., Barry, F., . . . Ritter, T. (2012). Immunogenicity of allogeneic mesenchymal stem cells. Journal of Cellular and Molecular Medicine, 16(9), 2094-2103.en_IE
dc.descriptionJournal articleen_IE
dc.description.abstractMesenchymal stem cells (MSCs) inhibit proliferation of allogeneic T cells and express low levels of major histocompatibility complex class I (MHCI), MHCII and vascular adhesion molecule-1 (VCAM-1). We investigated whether their immunosuppressive properties and low immunophenotype protect allogeneic rat MSCs against cytotoxic lysis in vitro and result in a reduced immune response in vivo. Rat MSCs were partially protected against alloantigen-specific cytotoxic T cells in vitro. However, after treatment with IFN-? and IL-1 beta, MSCs upregulated MHCI, MHCII and VCAM-1, and cytotoxic lysis was significantly increased. In vivo, allogeneic T cells but not allogeneic MSCs induced upregulation of the activation markers CD25 and CD71 as well as downregulation of CD62L on CD4+ T cells from recipient rats. However, intravenous injection of allo-MSCs in rats led to the formation of alloantibodies with the capacity to facilitate complement-mediated lysis, although IgM levels were markedly decreased compared with animals that received T cells. The allo-MSC induced immune response was sufficient to lead to significantly reduced survival of subsequently injected allo-MSCs. Interestingly, no increased immunogenicity of IFN-? stimulated allo-MSCs was observed in vivo. Both the loss of protection against cytotoxic lysis under inflammatory conditions and the induction of complement-activating antibodies will likely impact the utility of allogeneic MSCs for therapeutic applications.en_IE
dc.relation.ispartofJournal Of Cellular And Molecular Medicineen
dc.subjectMesenchymal stem cellsen_IE
dc.subjectAcute myocardial infarctionen_IE
dc.subjectProliferation in vitroen_IE
dc.subjectMarrow stromal cellsen_IE
dc.subjectVersus host diseaseen_IE
dc.subjectAcute renal failureen_IE
dc.subjectImmune responseen_IE
dc.subjectCardiac allograften_IE
dc.subjectGene therapyen_IE
dc.subjectNitric oxideen_IE
dc.subjectCalf serumen_IE
dc.titleImmunogenicity of allogeneic mesenchymal stem cellsen_IE
dc.local.contactThomas Ritter, School Of Medicine, Regenerative Medicine Institute, Biosciences, Dangan. 5329 Email:

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