dc.contributor.advisor | Morrison, Ciaran | |
dc.contributor.author | Kliszczak, Maciej | |
dc.date.accessioned | 2012-06-14T11:57:23Z | |
dc.date.available | 2013-01-21T13:10:03Z | |
dc.date.issued | 2011-06 | |
dc.identifier.uri | http://hdl.handle.net/10379/2861 | |
dc.description.abstract | Nse2/ Mms21 is an E3 SUMO ligase component of the Smc5/6 complex, which
plays multiple roles in maintaining genome stability. To study the functions of
the vertebrate Nse2 orthologue, we generated Nse2-deficient chicken DT40 cells.
Nse2 was dispensable for DT40 cell viability and required for efficient repair of
bulky DNA lesions, although double-strand break repair was unaffected.
Homologous recombination activities were reduced in Nse2-/-/- cells. Nse2
deficiency destabilised Smc5, but not Smc6. In rescue experiments, we found
that the SUMO ligase activity of Nse2 was required for an efficient response to
MMS-induced DNA damage, but not for Smc5 stability. Analysis of Nse2-/-/-
Smc5- clones, which were viable although slow-growing, showed that Smc5 and
Nse2 are not epistatic in DNA repair, suggesting that Smc5 and Nse2 have some
non-overlapping functions. | en_US |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | SMC proteins, | en_US |
dc.subject | Nse2 | en_US |
dc.subject | Mms21 | en_US |
dc.subject | DNA repair | en_US |
dc.subject | DT40 | en_US |
dc.subject | SUMO ligase | en_US |
dc.subject | Biochemistry | en_US |
dc.title | Functional dissection of the Smc5/6 complex: roles of the SUMO ligase Nse2/Mms21 in the maintenance of genome stability | en_US |
dc.type | Thesis | en_US |
dc.contributor.funder | College of Science Felllowship | en_US |
dc.local.note | Maciej Kliszczak has graduated from Wroclaw University of Technology with Engineer and Master of Science degrees in Biotechnology (Medicinal Chemistry) in 2007, defending thesis entitled "Responses of human cell lines to DNA damaging agents and to novel cancer growth inhibitors". In 2007, Maciej Kliszczak moved to Galway and started his Doctor of Philosophy Degree at National University of Ireland Galway under the guidance of Prof. Ciaran Morrison and defended his dissertation thesis in 2011. | en_US |
dc.local.final | Yes | en_US |
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