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Production and characterization of chemically cross-linked collagen scaffolds

dc.contributor.authorSallent, Ignacio
dc.contributor.authorCapella-Monsonís, Héctor
dc.contributor.authorZeugolis, Dimitrios I.
dc.date.accessioned2020-04-20T08:37:20Z
dc.date.available2020-04-20T08:37:20Z
dc.date.issued2019-03-07
dc.identifier.citationSallent I., Capella-Monsonís H., Zeugolis D.I. (2019) Production and Characterization of Chemically Cross-Linked Collagen Scaffolds. In: Sagi I., Afratis N. (eds) Collagen. Methods in Molecular Biology, vol 1944. Humana Press, New York, NYen_IE
dc.identifier.isbn978-1-4939-9095-5
dc.identifier.issn1940-6029
dc.identifier.urihttp://hdl.handle.net/10379/15885
dc.description.abstractChemical cross-linking of collagen-based devices is used as a means of increasing the mechanical stability and control the degradation rate upon implantation. Herein, we describe techniques to produce cross-linked with glutaraldehyde (GTA; amine terminal cross-linker), 4-arm polyethylene glycol succinimidyl glutarate (4SP; amine terminal cross-linker), diphenyl phosphoryl azide (DPPA; carboxyl terminal cross-linker) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC; carboxyl terminal cross-linker) collagen films. In addition, we provide protocols to characterise the biophysical (swelling), biomechanical (tensile) and biological (metabolic activity, proliferation and viability using human dermal fibroblasts and THP-1 macrophages) properties of the cross-linked collagen scaffolds.en_IE
dc.description.sponsorshipThis work has been supported by the Science Foundation Ireland, Career Development Award Programme (grant agreement number: 15/CDA/3629); Science Foundation Ireland and the European Regional Development Fund (grant agreement number: 13/RC/2073); and EU H2020, ITN award, Tendon Therapy Train Project (grant agreement number: 676338).en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherHumana Pressen_IE
dc.relation.ispartofCollagen, Methods and Protocolsen
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectCollagen cross-linkingen_IE
dc.subjectbiophysical characterisationen_IE
dc.subjectbiomechanical propertiesen_IE
dc.subjectcytotoxicityen_IE
dc.subjectmacrophage responseen_IE
dc.titleProduction and characterization of chemically cross-linked collagen scaffoldsen_IE
dc.typeBook chapteren_IE
dc.date.updated2020-04-18T19:17:43Z
dc.identifier.doi10.1007/978-1-4939-9095-5_3
dc.local.publishedsourcehttps://doi.org/10.1007/978-1-4939-9095-5_3en_IE
dc.description.peer-reviewedPeer reviewed
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderEuropean Regional Development Funden_IE
dc.contributor.funderHorizon 2020en_IE
dc.internal.rssid20667200
dc.local.contactIgnacio Sallent, Biomedical Sciences Building, Newcastle Road, Nui Galway. Email: i.sallent1@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionSUBMITTED
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Career Development Award/15/CDA/3629/IE/Tissue Engineered Nanoassemblies _ Advanced Biomimicry of Living Equivalents (Short Title: TENABLE)/en_IE
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/13/RC/2073/IE/C�RAM - Centre for Research in Medical Devices/en_IE
dcterms.projectinfo:eu-repo/grantAgreement/EC/H2020::MSCA-ITN-ETN/676338/EU/Engineering in vitro microenvironments for translation of cell-based therapies for tendon repair/Tendon Therapy Trainen_IE
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland