Phenotypic and functional heterogeneity of human intermediate monocytes based on HLA-DR expression
Connaughton, Eanna P.
Hanley, Shirley A.
Slevin, Stephanie M.
Eykelenboom, John K.
Lowndes, Noel F.
Griffin, Matthew D.
Dennedy, Michael C.
MetadataShow full item record
This item's downloads: 414 (view details)
Cited 7 times in Scopus (view citations)
Connaughton, Eanna P, Naicker, Serika, Hanley, Shirley A, Slevin, Stephanie M, Eykelenboom, John K, Lowndes, Noel F, O'Brien, Timothy, Ceredig, Rhodri, Griffin, Matthew D, Dennedy, Michael C. (2018). Phenotypic and functional heterogeneity of human intermediate monocytes based on HLA-DR expression. Immunology & Cell Biology, 96(7), 742-758. doi:10.1111/imcb.12032
Human blood monocytes are subclassified as classical, intermediate and nonclassical. In this study, it was shown that conventionally defined human intermediate monocytes can be divided into two distinct subpopulations with mid- and high-level surface expression of HLA-DR (referred to as DRmid and DRhi intermediate monocytes). These IM subpopulations were phenotypically and functionally characterized in healthy adult blood by flow cytometry, migration assays and lipoprotein uptake assays. Their absolute numbers and proportions were then compared in blood samples from obese and nonobese adults. DRmid and DRhi intermediate monocytes differentially expressed several proteins including CD62L, CD11a, CX3CR1 and CCR2. Overall, the DRmid intermediate monocytes surface profile more closely resembled that of classical monocytes while DRhi intermediate monocytes were more similar to nonclassical. However, in contrast to classical monocytes, DRmid intermediate monocytes migrated weakly to CCL2, had reduced intracellular calcium flux following CCR2 ligation and favored adherence to TNF alpha-activated endothelium over transmigration. In lipid uptake assays, DRmid intermediate monocytes demonstrated greater internalization of oxidized and acetylated low-density lipoprotein than DRhi intermediate monocytes. In obese compared to nonobese adults, proportions and absolute numbers of DRmid, but not DRhi intermediate monocytes, were increased in blood. The results are consistent with phenotypic and functional heterogeneity within the intermediate monocytes subset that may be of specific relevance to lipoprotein scavenging and metabolic health.