Browsing University of Galway Support Services by Subject "saccharomyces-cerevisiae"
Now showing items 1-20 of 29
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53bp1: function and mechanisms of focal recruitment
(Portland Press Ltd., 2009-08-01)53BP1 (p53-binding protein 1) is classified as a mediator/adaptor of the DNA-damage response, and is recruited to nuclear structures termed foci following genotoxic insult. In the present paper, we review the functions of ... -
Analysis of scc1-deficient cells defines a key metaphase role of vertebrate cohesin in linking sister kinetochores
(Wiley-Blackwell, 2004-02-01)Cleavage of the cohesin subunit Scc1p/Mcd1p/Rad21 permits sister chromatid separation and is considered to trigger anaphase onset. It has also been suggested that the cohesin complex is essential for chromosome condensation ... -
Atr activates the s-m checkpoint during unperturbed growth to ensure sufficient replication prior to mitotic onset
(Elsevier BV, 2013-11-01)Cells must accurately replicate and segregate their DNA once per cell cycle in order to successfully transmit genetic information. During S phase in the presence of agents that cause replication stress, ATR-dependent ... -
Budding yeast rad9 is an atp-dependent rad53 activating machine
(Elsevier BV, 2001-07-01)We find budding yeast Rad9 in two distinct, large, and soluble complexes in cell extracts. The larger (greater than or equal to 850 kDa) complex, found in nondamaged cells, contains hypophosphorylated Rad9, whereas the ... -
Coordinated regulation of replication protein a activities by its subunits p14 and p32
(American Society for Biochemistry & Molecular Biology (ASBMB), 2004-06-17)The heterotrimeric replication protein A (RPA) has multiple essential activities in eukaryotic DNA metabolism and in signaling pathways. Despite extensive analyses, the functions of the smallest RPA subunit p14 are still ... -
Distinctive activities of dna polymerases during human dna replication
(Wiley-Blackwell, 2006-07-01)The contributions of human DNA polymerases (pols) alpha, delta and epsilon during S-phase progression were studied in order to elaborate how these enzymes co-ordinate their functions during nuclear DNA replication. Pol ... -
Dna polymerase ε associates with the elongating form of rna polymerase ii and nascent transcripts
(Wiley-Blackwell, 2006-11-15)DNA polymerase epsilon co-operates with polymerases alpha and delta in the replicative DNA synthesis of eukaryotic cells. We describe here a specific physical interaction between DNA polymerase epsilon and RNA polymerase ... -
Extracellular vesicles from paracoccidioides pathogenic species transport polysaccharide and expose ligands for dc-sign receptors
(Springer Nature, 2015-09-21)Extracellular vesicles (EVs) mediate non-conventional transport of molecules across the fungal cell wall. We aimed at describing the carbohydrate composition and surface carbohydrate epitopes of EVs isolated from the ... -
Fungal enzymes for bio-products from sustainable and waste biomass
(Elsevier BV, 2016-07-01)Lignocellulose, the most abundant renewable carbon source on earth, is the logical candidate to replace fossil carbon as the major biofuel raw material. Nevertheless, the technologies needed to convert lignocellulose into ... -
Heritability in the efficiency of nonsense-mediated mrna decay in humans
(Public Library of Science (PLoS), 2010-07-21)Background: In eukaryotes mRNA transcripts of protein-coding genes in which an intron has been retained in the coding region normally result in premature stop codons and are therefore degraded through the nonsense-mediated ... -
Increased sister chromatid cohesion and dna damage response factor localization at an enzyme-induced dna double-strand break in vertebrate cells
(Oxford University Press (OUP), 2009-08-21)The response to DNA damage in vertebrate cells involves successive recruitment of DNA signalling and repair factors. We used light microscopy to monitor the genetic dependencies of such localization to a single, induced ... -
Interplay of dna damage and cell cycle signaling at the level of human replication protein a
(Elsevier BV, 2014-09-01) -
Mechanisms for atp-dependent chromatin remodelling: the means to the end
(Wiley-Blackwell, 2011-09-08)Chromatin remodelling is the ATP-dependent change in nucleosome organisation driven by Snf2 family ATPases. The biochemistry of this process depends on the behaviours of ATP-dependent motor proteins and their dynamic ... -
Mitochondrial malate dehydrogenase from the thermophilic, filamentous fungus talaromyces emersonii
(Wiley-Blackwell, 2004-07-15)Mitochondrial malate dehydrogenase (m-MDH; EC 1.1.1.37), from mycelial extracts of the thermophilic, aerobic fungus Talaromyces emersonii, was purified to homogeneity by sequential hydrophobic interaction and biospecific ... -
Mrc1, tof1 and csm3 inhibit cag·ctg repeat instability by at least two mechanisms
(Elsevier BV, 2008-04-01)Trinucleotide repeats frequently expand and contract in humans and model organisms. Protein factors that modulate this process have been found by candidate gene approaches or mutant screens for increased expansion rates. ... -
Mutsβ and histone deacetylase complexes promote expansions of trinucleotide repeats in human cells
(Oxford University Press (OUP), 2012-08-30)Trinucleotide repeat (TNR) expansions cause at least 17 heritable neurological diseases, including Huntington's disease. Expansions are thought to arise from abnormal processing of TNR DNA by specific trans-acting proteins. ... -
Regulation of cdc45 in the cell cycle and after dna damage
(Portland Press Ltd., 2009-08-01)The Cdc (cell division cycle) 45 protein has a central role in the regulation of the initiation and elongation stages of eukaryotic chromosomal DNA replication. In addition, it is the main target for a Chk1 (checkpoint ... -
Regulation of the dna damage response and gene expression by the dot1l histone methyltransferase and the 53bp1 tumour suppressor
(Public Library of Science (PLoS), 2011-02-24)Background: Dot1L, a histone methyltransferase that targets histone H3 lysine 79 (H3K79), has been implicated in gene regulation and the DNA damage response although its functions in these processes remain poorly ... -
Repression of g1/s transcription is mediated via interaction of the gtb motifs of nrm1 and whi5 with swi6
(American Society for Microbiology, 2013-02-04)In Saccharomyces cerevisiae, G(1)/S transcription factors MBF and SBF regulate a large family of genes important for entry to the cell cycle and DNA replication and repair. Their regulation is crucial for cell viability, ... -
Roles of vertebrate smc5 in sister chromatid cohesion and homologous recombinational repair
(American Society for Microbiology, 2011-01-18)The structural maintenance of chromosomes (Smc) family members Smc5 and Smc6 are both essential in budding and fission yeasts. Yeast smc5/6 mutants are hypersensitive to DNA damage, and Smc5/6 is recruited to HO-induced ...