Browsing by Author "Gerlach, Jared Q."
Now showing items 1-14 of 14
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Campylobacter jejuni strain discrimination and temperature-dependent glycome expression profiling by lectin microarray
Kilcoyne, Michelle; Twomey, Marcus E.; Gerlach, Jared Q.; Kane, Marian; Moran, Anthony P.; Joshi, Lokesh (Elsevier, 2014-02-12)Gram-negative Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans worldwide and the most frequently identified infectious trigger in patients developing Guillain-Barré syndrome (GBS). While ... -
Construction of a Natural Mucin Microarray and Interrogation for Biologically Relevant Glyco-Epitopes
Kilcoyne, Michelle; Gerlach, Jared Q.; Kane, Marian; Joshi, Lokesh (2012)Mucins are the principal components of mucus, and mucin glycosylation has important roles in defense, microbial adhesion, immunomodulation, inflammation, and cancer. Mucin expression and glycosylation are dynamic, responding ... -
Differential release of high mannose structural isoforms by fungal and bacterial endo-β-N-acetylglucosaminidases
Gerlach, Jared Q.; Kilcoyne, Michelle; Farrell, Mark P.; Kane, Marian; Joshi, Lokesh (Royal Society of Chemistry, 2012-02-28)Endo-beta-N-acetylglucosaminidases (ENGases) are widely used to remove N-linked oligosaccharides from glycoproteins for glycomic and proteomic studies and biopharmaceutical processes. Although several ENGases are widely ... -
Distinctive surface glycosylation patterns associated with mouse and human cd4+ regulatory t cells and their suppressive function
Cabral, Joana; Hanley, Shirley A.; Gerlach, Jared Q.; O’Leary, Neil; Cunningham, Stephen; Ritter, Thomas; Ceredig, Rhodri; Joshi, Lokesh; Griffin, Matthew D. (Frontiers Media SA, 2017-08-21)Regulatory T-cells (Treg) are essential for maintaining immune homeostasis and tolerance. Surface glycosylation is ubiquitous on mammalian cells and regulates diverse biological processes. While it is currently well accepted ... -
Examination of oestrus-dependent alterations of bovine cervico-vaginal mucus glycosylation for potential as optimum fertilisation indicators
Le Berre, Marie; Gerlach, Jared Q.; Loughrey, Catherine; Creavin, Aileen; Pluta, Katarzyna; Gallagher, Mary; Carrington, Stephen D.; Joshi, Lokesh; Kilcoyne, Michelle (Royal Society of Chemistry, 2021-03-09)Oestrus is the period in the sexual cycle of female mammals during which ovulation occurs, where they become most receptive to mating and are most fertile. Efficient detection of oestrus is a key component in successful ... -
Extracellular vesicles from paracoccidioides pathogenic species transport polysaccharide and expose ligands for dc-sign receptors
da Silva, Roberta Peres; Heiss, Christian; Black, Ian; Azadi, Parastoo; Gerlach, Jared Q.; Travassos, Luiz R.; Joshi, Lokesh; Kilcoyne, Michelle; Puccia, Rosana (Springer Nature, 2015-09-21)Extracellular vesicles (EVs) mediate non-conventional transport of molecules across the fungal cell wall. We aimed at describing the carbohydrate composition and surface carbohydrate epitopes of EVs isolated from the ... -
Fasciola hepaticasurface tegument: glycoproteins at the interface of parasite and host
Ravidà, Alessandra; Cwiklinski, Krystyna; Aldridge, Allison M.; Clarke, Paul; Thompson, Roisin; Gerlach, Jared Q.; Kilcoyne, Michelle; Hokke, Cornelis H.; Dalton, John P.; O'Neill, Sandra M. (American Society for Biochemistry & Molecular Biology (ASBMB), 2016-07-27)Fasciola hepatica, commonly known as liver fluke, is a trematode that causes Fasciolosis in ruminants and humans. The outer tegumental coat of F. hepatica (FhTeg) is a complex metabolically active biological matrix that ... -
Periodic acid-Schiff's reagent assay for carbohydrates in a microtiter plate format
Kilcoyle, Michelle; Gerlach, Jared Q.; Farrell, Mark P.; Joshi, Lokesh (2011)Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low neutral sugar content. We have adapted and optimised ... -
Rapid screening for specific glycosylation and pathogen interactions on a 78 species avian egg white glycoprotein microarray
Utratna, Marta; Annuk, Heidi; Gerlach, Jared Q.; Lee, Yuan C.; Kane, Marian; Kilcoyne, Michelle; Joshi, Lokesh (Nature Research, 2017-07-25)There is an urgent need for discovery of novel antimicrobials and carbohydrate-based anti-adhesive strategies are desirable as they may not promote resistance. Discovery of novel anti-adhesive molecules from natural product ... -
Regulating immunogenicity and tolerogenicity of bone marrow-derived dendritic cells through modulation of cell surface glycosylation by dexamethasone treatment
Lynch, Kevin; Treacy, Oliver; Gerlach, Jared Q.; Annuk, Heidi; Lohan, Paul; Cabral, Joana; Joshi, Lokesh; Ryan, Aideen E.; Ritter, Thomas (Frontiers Media, 2017-10-30)Dendritic cellular therapies and dendritic cell vaccines show promise for the treatment of autoimmune diseases, the prolongation of graft survival in transplantation, and in educating the immune system to fight cancers. ... -
Regulating immunogenicity and tolerogenicity of bone marrow-derived dendritic cells through modulation of cell surface glycosylation by dexamethasone treatment
Lynch, Kevin; Treacy, Oliver; Gerlach, Jared Q.; Annuk, Heidi; Lohan, Paul; Cabral, Joana; Joshi, Lokesh; Ryan, Aideen E.; Ritter, Thomas (Frontiers Media SA, 2017-10-30)Dendritic cellular therapies and dendritic cell vaccines show promise for the treatment of autoimmune diseases, the prolongation of graft survival in transplantation, and in educating the immune system to fight cancers. ... -
Surface chemistry and linker effects on lectin-carbohydrate recognition for glycan microarrays
Kilcoyne, Michelle; Gerlach, Jared Q.; Kane, Marian; Joshi, Lokesh (2012)Glycan microarrays are an increasingly utilised tool for analysis of protein-carbohydrate interactions and a variety of glycan-containing molecules and slide chemistries have been used to array carbohydrates on microarray ... -
Surface glycosylation profiles of urine extracellular vesicles
Gerlach, Jared Q.; Krüger, Anja; Gallogly, Susan; Hanley, Shirley A.; Hogan, Marie C.; Ward, Christopher J.; Joshi, Lokesh; Griffin, Matthew D. (Public Library of Science (PLoS), 2013-09-19)Urinary extracellular vesicles (uEVs) are released by cells throughout the nephron and contain biomolecules from their cells of origin. Although uEV-associated proteins and RNA have been studied in detail, little information ... -
Temporal alterations in the bovine buttermilk glycome from parturition to milk maturation
Ross, Sarah A.; Gerlach, Jared Q.; Gill, Satbir K.; Lane, Jonathan A.; Kilcoyne, Michelle; Hickey, Rita M.; Joshi, Lokesh (Elsevier BV, 2016-11-01)