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dc.contributor.authorDowling, Enda P.
dc.contributor.authorRonan, William
dc.contributor.authorMcGarry, J. Patrick
dc.identifier.citationDowling, Enda P., Ronan, William, & McGarry, J. Patrick. (2013). Computational investigation of in situ chondrocyte deformation and actin cytoskeleton remodelling under physiological loading. Acta Biomaterialia, 9(4), 5943-5955. doi:
dc.description.abstractPrevious experimental studies have determined local strain fields for both healthy and degenerate cartilage tissue during mechanical loading. However, the biomechanical response of chondrocytes in situ, in particular the response of the actin cytoskeleton to physiological loading conditions, is poorly understood. In the current study a three-dimensional (3-D) representative volume element (RVE) for cartilage tissue is created, comprising a chondrocyte surrounded by a pericellular matrix and embedded in an extracellular matrix. A 3-D active modelling framework incorporating actin cytoskeleton remodelling and contractility is implemented to predict the biomechanical behaviour of chondrocytes. Physiological and abnormal strain fields, based on the experimental study of Wong and Sah (J. Orthop. Res. 2010; 28: 1554-1561), are applied to the RVE. Simulations demonstrate that the presence of a focal defect significantly affects cellular deformation, increases the stress experienced by the nucleus, and alters the distribution of the actin cytoskeleton. It is demonstrated that during dynamic loading cyclic tension reduction in the cytoplasm causes continuous dissociation of the actin cytoskeleton. In contrast, during static loading significant changes in cytoplasm tension are not predicted and hence the rate of dissociation of the actin cytoskeleton is reduced. It is demonstrated that chondrocyte behaviour is affected by the stiffness of the pericellular matrix, and also by the anisotropy of the extracellular matrix. The findings of the current study are of particular importance in understanding the biomechanics underlying experimental observations such as actin cytoskeleton dissociation during the dynamic loading of chondrocytes. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.en_IE
dc.description.sponsorshipIrish Research Council for Science, Engineering and Technology postgraduate scholarship under the EMBARK initiative, and by the Science Foundation Ireland Research Frontiers Programme (SFI-RFP/ENM1726).en_IE
dc.relation.ispartofActa Biomaterialiaen
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.subjectCell mechanicsen_IE
dc.subjectActin cytoskeleton remodellingen_IE
dc.subjectFinite element modellingen_IE
dc.subjectArticular cartilage defectsen_IE
dc.subjectAtomic force microscopyen_IE
dc.subjectPericellular matrixen_IE
dc.subjectMechanical propertiesen_IE
dc.subjectGene expressionen_IE
dc.subjectFinite elementen_IE
dc.subjectBiomechanical propertiesen_IE
dc.subjectMicropipette aspirationen_IE
dc.subjectHydrostatic pressureen_IE
dc.subjectDynamic compressionen_IE
dc.subjectMechanical engineeringen_IE
dc.titleComputational investigation of in situ chondrocyte deformation and actin cytoskeleton remodelling under physiological loadingen_IE
dc.local.contactWilliam Ronan, Mechanical Engineering, School Of Engineering, Nui Galway. Email:

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Attribution-NonCommercial-NoDerivs 3.0 Ireland
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