Show simple item record

dc.contributor.authorKilcoyne, Michelle
dc.contributor.authorGerlach, Jared Q.
dc.contributor.authorKane, Marian
dc.contributor.authorJoshi, Lokesh
dc.date.accessioned2013-07-26T10:31:51Z
dc.date.available2013-07-26T10:31:51Z
dc.date.issued2012
dc.identifier.citationKilcoyne, M,Gerlach, JQ,Kane, M,Joshi, L (2012) 'Surface chemistry and linker effects on lectin-carbohydrate recognition for glycan microarrays'. Analytical Methods, 4 :2721-2728.en_US
dc.identifier.urihttp://hdl.handle.net/10379/3544
dc.description.abstractGlycan microarrays are an increasingly utilised tool for analysis of protein-carbohydrate interactions and a variety of glycan-containing molecules and slide chemistries have been used to array carbohydrates on microarray surfaces. Slide surface chemistry can have significant impact on the ligand presentation, background noise, spot size and morphology and reproducibility of the arrayed molecules, which in turn impacts upon lectin-carbohydrate recognition. The linker used to attach the carbohydrate to the molecular scaffold is another variable in ligand presentation. To evaluate these effects, three different microarray surface chemistries were arrayed with the same mono-and disaccharide neoglycoconjugates and natural glycoproteins and incubated with four well-characterised plant lectins. Analogues of three monosaccharide neoglycoconjugates, with two common linkers each, were included in the test group to evaluate the linker effect on lectin recognition. Based on lowest background noise, expected lectin-ligand interaction, good spot morphology and best reproducibility, the three-dimensional hydrogel slide surface proved most suitable for lectin interrogation of carbohydrate ligands, and the more flexible phenylisothiocyanate linker afforded greater recognition of the carbohydrates by the relevant lectins.en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.relation.ispartofAnalytical Methodsen
dc.subjectProtein interactionsen_US
dc.subjectBinding-proteinsen_US
dc.subjectConcanavalin-aen_US
dc.subjectSpecificityen_US
dc.subjectOligosaccharideen_US
dc.subjectGlycosylationen_US
dc.subjectConstructionen_US
dc.subjectGlycoproteinen_US
dc.subjectAntibodiesen_US
dc.subjectPrincipleen_US
dc.titleSurface chemistry and linker effects on lectin-carbohydrate recognition for glycan microarraysen_US
dc.typeArticleen_US
dc.date.updated2013-07-24T12:18:29Z
dc.identifier.doiDOI 10.1039/c2ay25532d
dc.local.publishedsourcehttp://dx.doi.org/10.1039/c2ay25532den_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid2469054
dc.local.contactLokesh Joshi, School Of Natural Sciences, Nui Galway. 5768 Email: lokesh.joshi@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
nui.item.downloads989


Files in this item

Attribution-NonCommercial-NoDerivs 3.0 Ireland
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. Please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.

The following license files are associated with this item:

Thumbnail

This item appears in the following Collection(s)

Show simple item record