dc.contributor.author | Owens, Peter | |
dc.contributor.author | Phillipson, Nigel | |
dc.contributor.author | Perumal, Jayakumar | |
dc.contributor.author | O’Connor, Gerard | |
dc.contributor.author | Olivo, Malini | |
dc.date.accessioned | 2018-09-20T16:20:56Z | |
dc.date.available | 2018-09-20T16:20:56Z | |
dc.date.issued | 2015-10-28 | |
dc.identifier.citation | Owens, Peter; Phillipson, Nigel; Perumal, Jayakumar; O’Connor, Gerard; Olivo, Malini (2015). Sensing of p53 and egfr biomarkers using high efficiency sers substrates. Biosensors 5 (4), 664-677 | |
dc.identifier.issn | 2079-6374 | |
dc.identifier.uri | http://hdl.handle.net/10379/13383 | |
dc.description.abstract | In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein-antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration. | |
dc.publisher | MDPI AG | |
dc.relation.ispartof | Biosensors | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | raman spectroscopy | |
dc.subject | protein sensing | |
dc.subject | surface-enhanced raman scattering | |
dc.subject | biomarker | |
dc.title | Sensing of p53 and egfr biomarkers using high efficiency sers substrates | |
dc.type | Article | |
dc.identifier.doi | 10.3390/bios5040664 | |
dc.local.publishedsource | http://www.mdpi.com/2079-6374/5/4/664/pdf | |
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