Sensing of p53 and egfr biomarkers using high efficiency sers substrates
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Owens, Peter; Phillipson, Nigel; Perumal, Jayakumar; O’Connor, Gerard; Olivo, Malini (2015). Sensing of p53 and egfr biomarkers using high efficiency sers substrates. Biosensors 5 (4), 664-677
In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein-antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration.