dc.contributor.author | Bishop, Gregory W. | |
dc.contributor.author | Satterwhite-Warden, Jennifer E. | |
dc.contributor.author | Bist, Itti | |
dc.contributor.author | Chen, Eric | |
dc.contributor.author | Rusling, James F. | |
dc.date.accessioned | 2018-09-20T16:01:07Z | |
dc.date.available | 2018-09-20T16:01:07Z | |
dc.date.issued | 2016-02-26 | |
dc.identifier.citation | Bishop, Gregory W. Satterwhite-Warden, Jennifer E.; Bist, Itti; Chen, Eric; Rusling, James F. (2016). Electrochemiluminescence at bare and dna-coated graphite electrodes in 3d-printed fluidic devices. ACS Sensors 1 (2), 197-202 | |
dc.identifier.issn | 2379-3694,2379-3694 | |
dc.identifier.uri | http://hdl.handle.net/10379/10444 | |
dc.description.abstract | Clear plastic fluidic devices with ports for incorporating electrodes to enable electrochemiluminescence (ECL) measurements were prepared using a low-cost, desktop three-dimensional (3D) printer based on stereolithography. Electrodes consisted of 0.5 mm pencil graphite rods and 0.5 mm silver wires inserted into commercially available 1/4 in -28 threaded fittings. A bioimaging system equipped with a CCD camera was used to measure ECL generated at electrodes and small arrays using 0.2 M phosphate buffer solutions containing tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate ([Ru(bpy)(3)](2+)) with 100 mM tri-n-propylamine (TPA) as the coreactant. ECL signals produced at pencil graphite working electrodes were linear with respect to [Ru(bpy)(3)](2+) concentration for 9-900 mu M [Ru(bpy)(3)](2+). The detection limit was found to be 7 mu M using the CCD camera with exposure time set at 10 s. Electrode-to-electrode ECL signals varied by +/- 7.5%. Device performance was further evaluated using pencil graphite electrodes coated with multilayer poly(diallyldimethylammonium chloride) (PDDA)/DNA films. In these experiments, ECL resulted from the reaction of [Ru(bpy)(3)](3+) with guanines of DNA. ECL produced at these thin-film electrodes was linear with respect to [Ru(bpy)(3)](2+) concentration from 180 to 800 mu M. These studies provide the first demonstration of ECL measurements obtained using a 3D-printed closed-channel fluidic device platform. The affordable, high-resolution 3D printer used in these studies enables easy, fast, and adaptable prototyping of fluidic devices capable of incorporating electrodes for measuring ECL. | |
dc.publisher | American Chemical Society (ACS) | |
dc.relation.ispartof | ACS Sensors | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | 3d printed fluidics | |
dc.subject | electrochemiluminescence | |
dc.subject | stereolithography | |
dc.subject | DNA oxidation | |
dc.subject | biosensing | |
dc.subject | microfluidic devices | |
dc.subject | electrogenerated chemiluminescence | |
dc.subject | versatile | |
dc.subject | films | |
dc.title | Electrochemiluminescence at bare and dna-coated graphite electrodes in 3d-printed fluidic devices | |
dc.type | Article | |
dc.identifier.doi | 10.1021/acssensors.5b00156 | |
dc.local.publishedsource | http://europepmc.org/articles/pmc4847733?pdf=render | |
nui.item.downloads | 0 | |