Browsing by Author "Crowley, Peter B."

Now showing items 1-11 of 11

    • Calixarene-mediated assembly of a small antifungal protein 

      Alex, Jimi M.; Rennie, Martin L.; Engilberge, Sylvain; Lehoczki, Gábor; Dorottya, Hajdu; Fizil, Ádám; Batta, Gyula; Crowley, Peter B. (International Union of Crystallography, 2019-02-05)
      Synthetic macrocycles such as calixarenes and cucurbiturils are increasingly applied as mediators of protein assembly and crystallization. The macrocycle can facilitate assembly by providing a surface on which two or more ...

    • Grasping the nature of the cell interior: fromphysiological chemistrytochemical biology 

      Kyne, Ciara; Crowley, Peter B. (Wiley-Blackwell, 2016-05-11)
      Current models of the cell interior emphasise its crowded, chemically complex and dynamically organised structure. Although the chemical composition of cells is known, the cooperative intermolecular interactions that govern ...

    • Noncovalent pegylation via lectin–glycopolymer interactions 

      Antonik, Paweł M.; Eissa, Ahmed M.; Round, Adam R.; Cameron, Neil R.; Crowley, Peter B. (American Chemical Society (ACS), 2016-08-08)
      PEGylation, the covalent modification of proteins with polyethylene glycol, is an abundantly used technique to improve the pharmacokinetics of therapeutic proteins. The drawback with this methodology is that the covalently ...

    • Physicochemical properties of cells and their effects on intrinsically disordered proteins (idps) 

      Theillet, Francois-Xavier; Binolfi, Andres; Frembgen-Kesner, Tamara; Hingorani, Karan; Sarkar, Mohona; Kyne, Ciara; Li, Conggang; Crowley, Peter B.; Gierasch, Lila; Pielak, Gary J.; Elcock, Adrian H.; Gershenson, Anne; Selenko, Philipp (American Chemical Society (ACS), 2014-07-09)

    • Protein charge determination and implications for interactions in cell extracts 

      Kyne, Ciara; Jordon, Kiara; Filoti, Dana I.; Laue, Thomas M.; Crowley, Peter B. (Wiley-Blackwell, 2016-12-01)
      Decades of dilute-solution studies have revealed the influence of charged residues on protein stability, solubility and stickiness. Similar characterizations are now required in physiological solutions to understand the ...

    • Protein interactions in physiological environments 

      Kyne, Ciara (2016-06-03)
      The interactions of three proteins (ΔTat-GB1, cytochrome c and flavodoxin) were explored in Escherichia coli cells and extracts. The results indicate features of the physicochemical basis for protein assembly in the complex ...

    • Specific ion effects on macromolecular interactions inescherichia coliextracts 

      Kyne, Ciara; Ruhle, Brian; Gautier, Virginie W.; Crowley, Peter B. (Wiley-Blackwell, 2014-12-30)
      Protein characterization in situ remains a major challenge for protein science. Here, the interactions of Tat-GB1 in Escherichia coli cell extracts were investigated by NMR spectroscopy and size exclusion chromatography ...

    • Structural study of a small molecule receptor bound to dimethyllysine in lysozyme 

      McGovern, Róise E.; Snarr, Brendan D.; Lyons, Joseph A.; McFarlane, James; Whiting, Amanda L.; Paci, Irina; Hof, Fraser; Crowley, Peter B. (Royal Society of Chemistry (RSC), 2015-01-01)
      Lysine is a ubiquitous residue on protein surfaces. Post translational modifications of lysine, including methylation to the mono-, di- or trimethylated amine result in chemical and structural alterations that have major ...

    • Supramolecular stacking in a high Z′ calix[8]arene–porphyrin assembly 

      Alex, Jimi M.; McArdle, Patrick; Crowley, Peter B. (Royal Society of Chemistry, 2019-11-15)
      A co-crystal structure of sulfonato-calix[8]arene (sclx8) and trimethylanilinium-porphyrin (tmap) at 1.0 Å resolution is reported. The oppositely charged macrocycles formed an alternating stacked assembly. Crystal packing ...

    • A thermodynamic model of auto-regulated protein assembly by a supramolecular scaffold 

      Rennie, Martin L.; Crowley, Peter B. (Wiley, 2019-03-12)
      Ligand-mediated regulation of protein assembly occurs frequently in different cellular contexts. Auto-regulated assembly, where a ligand acts as its own competitive inhibitor, provides a mechanism for exquisite control of ...

    • Tuning protein frameworks via auxiliary supramolecular interactions 

      Engilberge, Sylvain; Rennie, Martin L.; Dumon, Elise; Crowley, Peter B. (American Chemical Society, 2019-09-06)
      Protein crystals with their precise, periodic array of functional building blocks have potential applications in biomaterials, sensing, and catalysis. This paper describes how a highly porous crystalline framework of a ...