Browsing by Subject "protein-a"
Now showing items 1-4 of 4
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A novel igy-aptamer hybrid system for cost-effective detection of seb and its evaluation on food and clinical samples
(Springer Nature, 2015-10-19)In the present study, we introduce a novel hybrid sandwich-ALISA employing chicken IgY and ssDNA aptamers for the detection of staphylococcal enterotoxin B (SEB). Cloning, expression and purification of the full length ... -
C-jun n-terminal kinase-mediated rad18 phosphorylation facilitates pol recruitment to stalled replication forks
(American Society for Cell Biology (ASCB), 2012-03-28)The E3 ubiquitin ligase Rad18 chaperones DNA polymerase eta (Pol eta) to sites of UV-induced DNA damage and monoubiquitinates proliferating cell nuclear antigen (PCNA), facilitating engagement of Pol eta with stalled ... -
Dna polymerase η modulates replication fork progression and dna damage responses in platinum-treated human cells
(Springer Nature, 2013-11-20)Human cells lacking DNA polymerase eta (pol eta) are sensitive to platinum-based cancer chemotherapeutic agents. Using DNA combing to directly investigate the role of pol eta in bypass of platinum-induced DNA lesions in ... -
Host-specific replication of bk virus dna in mouse cell extracts is independently controlled by dna polymerase -primase and inhibitory activities
(American Society for Microbiology, 2010-04-14)The activation of the human polyomavirus BK causes polyomavirus-associated nephropathy in immuno-compromised humans. Studies of the virus have been restricted since the virus DNA replication is species specific. Cell-based ...