Browsing by Subject "amplification"

Now showing items 1-19 of 19

    • Abnormal centrosomal structure and duplication in cep135-deficient vertebrate cells 

      Inanc, B.; Putz, M.; Lalor, P.; Dockery, P.; Kuriyama, R.; Gergely, F.; Morrison, C. G. (American Society for Cell Biology (ASCB), 2013-07-17)
      Centrosomes are key microtubule-organizing centers that contain a pair of centrioles, conserved cylindrical, microtubule-based structures. Centrosome duplication occurs once per cell cycle and relies on templated centriole ...

    • Automated multiplexed ecl immunoarrays for cancer biomarker proteins 

      Kadimisetty, Karteek; Malla, Spundana; Sardesai, Naimish P.; Joshi, Amit A.; Faria, Ronaldo C.; Lee, Norman H.; Rusling, James F. (American Chemical Society (ACS), 2015-04-21)
      Point-of-care diagnostics based on multiplexed protein measurements face challenges of simple, automated, low-cost, and high-throughput operation-with high sensitivity. Herein, we describe an automated, microprocessor-controlled ...

    • Clinically applicable inhibitors impacting genome stability 

      Prakash, Anu; Garcia-Moreno, Juan; Brown, James; Bourke, Emer (MDPI AG, 2018-05-13)
      Advances in technology have facilitated the molecular profiling (genomic and transcriptomic) of tumours, and has led to improved stratification of patients and the individualisation of treatment regimes. To fully realize ...

    • Comparative rna expression analyses from small-scale, single-donor platelet samples1 

      HILLMANN, A. G.; HARMON, S.; PARK, S. D. E.; O'BRIEN, J.; SHIELDS, D. C.; Kenny, D. (Wiley-Blackwell, 2006-02-01)
      Background: Comparisons of platelet RNAs could provide crucial information on platelet function, thrombopoiesis and the etiology of megakaryocyte (MK) or platelet disorders. Objectives: We developed a method for stringent ...

    • Detection of aeromonas salmonicida in wild atlantic salmon using a specific dna probe test 

      Mooney, J; Powell, E; Clabby, C; Powell, R (Inter-Research Science Center, 1995-01-01)
      The presence of Aeromonas salmonicida, the etiological agent of furunculosis, was examined using polymerase chain reaction (PCR) and DNA probe methodology in wild Atlantic salmon Salmo salar taken from 3 Irish river systems. ...

    • Development of a real-time multiplex pcr assay for the detection of multiple salmonella serotypes in chicken samples 

      O'Regan, Edel; McCabe, Evonne; Burgess, Catherine; McGuinness, Sheila; Barry, Thomas; Duffy, Geraldine; Whyte, Paul; Fanning, Séamus (Springer Nature, 2008-01-01)
      Background: A real-time multiplex PCR assay was developed for the detection of multiple Salmonella serotypes in chicken samples. Poultry-associated serotypes detected in the assay include Enteritidis, Gallinarum, Typhimurium, ...

    • Dna damage induces chk1-dependent threonine-160 phosphorylation and activation of cdk2 

      Bourke, E; Brown, J A L; Takeda, S; Hochegger, H; Morrison, C G (Springer Nature, 2009-10-19)
      Abnormal centrosome numbers arise in tumours and can cause multipolar mitoses and genome instability. Cdk2 controls normal centrosome duplication, but Chk1-dependent centrosome amplification also occurs after DNA damage. ...

    • Genetic diversity of the fish pathogen aeromonas salmonicida demonstrated by random amplified polymorphic dna and pulsed-field gel electrophoresis analyses 

      O'hIci, B; Olivier, G; Powell, R (Inter-Research Science Center, 2000-01-01)
      The current taxonomy of Aeromonas salmonicida includes 4 subspecies. A. salmonicida subsp. salmonicida is associated with salmonid furunculosis, and A. salmonicida subsp, achromogenes, A. salmonicida subsp. masoucida, and ...

    • Genetic variation in mitotic regulatory pathway genes is associated with breast tumor grade 

      Purrington, Kristen S.; Slettedahl, Seth; Bolla, Manjeet K.; Michailidou, Kyriaki; Czene, Kamila; Nevanlinna, Heli; Bojesen, Stig E.; Andrulis, Irene L.; Cox, Angela; Hall, Per; Carpenter, Jane; Yannoukakos, Drakoulis; Haiman, Christopher A.; Fasching, Peter A.; Mannermaa, Arto; Winqvist, Robert; Brenner, Hermann; Lindblom, Annika; Chenevix-Trench, Georgia; Benitez, Javier; Swerdlow, Anthony; Kristensen, Vessela; Guénel, Pascal; Meindl, Alfons; Darabi, Hatef; Eriksson, Mikael; Fagerholm, Rainer; Aittomäki, Kristiina; Blomqvist, Carl; Nordestgaard, Børge G.; Nielsen, Sune F.; Flyger, Henrik; Wang, Xianshu; Olswold, Curtis; Olson, Janet E.; Mulligan, Anna Marie; Knight, Julia A.; Tchatchou, Sandrine; Reed, Malcolm W.R.; Cross, Simon S.; Liu, Jianjun; Li, Jingmei; Humphreys, Keith; Clarke, Christine; Scott, Rodney; Fostira, Florentia; Fountzilas, George; Konstantopoulou, Irene; Henderson, Brian E.; Schumacher, Fredrick; Le Marchand, Loic; Ekici, Arif B.; Hartmann, Arndt; Beckmann, Matthias W.; Hartikainen, Jaana M.; Kosma, Veli-Matti; Kataja, Vesa; Jukkola-Vuorinen, Arja; Pylkäs, Katri; Kauppila, Saila; Dieffenbach, Aida Karina; Stegmaier, Christa; Arndt, Volker; Margolin, Sara; Balleine, Rosemary; Arias Perez, Jose Ignacio; Pilar Zamora, M.; Menéndez, Primitiva; Ashworth, Alan; Jones, Michael; Orr, Nick; Arveux, Patrick; Kerbrat, Pierre; Truong, Thérèse; Bugert, Peter; Toland, Amanda E.; Ambrosone, Christine B.; Labrèche, France; Goldberg, Mark S.; Dumont, Martine; Ziogas, Argyrios; Lee, Eunjung; Dite, Gillian S.; Apicella, Carmel; Southey, Melissa C.; Long, Jirong; Shrubsole, Martha; Deming-Halverson, Sandra; Ficarazzi, Filomena; Barile, Monica; Peterlongo, Paolo; Durda, Katarzyna; Jaworska-Bieniek, Katarzyna; Tollenaar, Robert A.E.M.; Seynaeve, Caroline; Brüning, Thomas; Ko, Yon-Dschun; Van Deurzen, Carolien H.M.; Martens, John W.M.; Kriege, Mieke; Figueroa, Jonine D.; Chanock, Stephen J.; Lissowska, Jolanta; Tomlinson, Ian; Kerin, Michael J.; Miller, Nicola; Schneeweiss, Andreas; Tapper, William J.; Gerty, Susan M.; Durcan, Lorraine; Mclean, Catriona; Milne, Roger L.; Baglietto, Laura; dos Santos Silva, Isabel; Fletcher, Olivia; Johnson, Nichola; Van'T Veer, Laura J.; Cornelissen, Sten; Försti, Asta; Torres, Diana; Rüdiger, Thomas; Rudolph, Anja; Flesch-Janys, Dieter; Nickels, Stefan; Weltens, Caroline; Floris, Giuseppe; Moisse, Matthieu; Dennis, Joe; Wang, Qin; Dunning, Alison M.; Shah, Mitul; Brown, Judith; Simard, Jacques; Anton-Culver, Hoda; Neuhausen, Susan L.; Hopper, John L.; Bogdanova, Natalia; Dörk, Thilo; Zheng, Wei; Radice, Paolo; Jakubowska, Anna; Lubinski, Jan; Devillee, Peter; Brauch, Hiltrud; Hooning, Maartje; García-Closas, Montserrat; Sawyer, Elinor; Burwinkel, Barbara; Marmee, Frederick; Eccles, Diana M.; Giles, Graham G.; Peto, Julian; Schmidt, Marjanka; Broeks, Annegien; Hamann, Ute; Chang-Claude, Jenny; Lambrechts, Diether; Pharoah, Paul D.P.; Easton, Douglas; Pankratz, V. Shane; Slager, Susan; Vachon, Celine M.; Couch, Fergus J. (Oxford University Press (OUP), 2014-06-13)
      Mitotic index is an important component of histologic grade and has an etiologic role in breast tumorigenesis. Several small candidate gene studies have reported associations between variation in mitotic genes and breast ...

    • High sensitivity carbon nanotube based electrochemiluminescence sensor array 

      Venkatanarayanan, Anita; Crowley, Karl; Lestini, Elena; Keyes, Tia E.; Rusling, James F.; Forster, Robert J. (Elsevier BV, 2012-01-01)

    • Inhibition of protein synthesis and jnk activation are not required for cell death induced by anisomycin and anisomycin analogues 

      Monaghan, David; O’Connell, Enda; Cruickshank, Faye L.; O’Sullivan, Barry; Giles, Francis J.; Hulme, Alison N.; Fearnhead, Howard O. (Elsevier BV, 2014-01-01)

    • Novel multiplex isothermal nucleic acid amplification technologies for the detection of bacterial meningitis associated pathogens 

      Higgins, Owen (NUI Galway, 2019-02-28)
      Infectious diseases, caused by pathogenic microorganisms such as bacteria, fungi or viruses, are a leading cause of significant global morbidity and mortality, with over 10 million associated deaths reported each year. ...

    • On-line protein capture on magnetic beads for ultrasensitive microfluidic immunoassays of cancer biomarkers 

      Otieno, Brunah A.; Krause, Colleen E.; Latus, Alina; Chikkaveeraiah, Bhaskara V.; Faria, Ronaldo C.; Rusling, James F. (Elsevier BV, 2014-03-01)

    • Phylogenetic relationships of the marine haplosclerida (phylum porifera) employing ribosomal (28s rrna) and mitochondrial (cox1, nad1) gene sequence data 

      Redmond, Niamh E.; Raleigh, Jean; van Soest, Rob W. M.; Kelly, Michelle; Travers, Simon A. A.; Bradshaw, Brian; Vartia, Salla; Stephens, Kelly M.; McCormack, Grace P. (Public Library of Science (PLoS), 2011-09-13)
      The systematics of the poriferan Order Haplosclerida (Class Demospongiae) has been under scrutiny for a number of years without resolution. Molecular data suggests that the order needs revision at all taxonomic levels. ...

    • Rapid real-time pcr detection of listeria monocytogenes in enriched food samples based on the ssra gene, a novel diagnostic target 

      O’ Grady, Justin; Sedano-Balbás, Sara; Maher, Majella; Smith, Terry; Barry, Thomas (Elsevier BV, 2008-02-01)

    • Simultaneous dna-rna extraction from coastal sediments and quantification of 16s rrna genes and transcripts by real-time pcr 

      Tatti, Enrico; McKew, Boyd A.; Whitby, Corrine; Smith, Cindy J. (MyJove Corporation, 2016-06-11)
      Real Time Polymerase Chain Reaction also known as quantitative PCR (q-PCR) is a widely used tool in microbial ecology to quantify gene abundances of taxonomic and functional groups in environmental samples. Used in combination ...

    • Single-wall carbon nanotube forest arrays for immunoelectrochemical measurement of four protein biomarkers for prostate cancer 

      Chikkaveeraiah, Bhaskara V.; Bhirde, Ashwin; Malhotra, Ruchika; Patel, Vyomesh; Gutkind, J. Silvio; Rusling, James F. (American Chemical Society (ACS), 2009-11-01)
      Protein arrays that measure multiple protein cancer biomarkers in clinical samples hold great promise for reliable early cancer detection. Herein, we report a prototype 4-unit electrochemical immunoarray based on single-wall ...

    • Systemic mirna-195 differentiates breast cancer from other malignancies and is a potential biomarker for detecting noninvasive and early stage disease 

      Heneghan, H. M.; Miller, N.; Kelly, R.; Newell, J.; Kerin, M. J. (Alphamed Press, 2010-06-24)
      Purpose. The potential of microRNAs (miRNAs) as novel tumor markers has been the focus of recent scrutiny because of their tissue specificity, stability, and association with clinicopathological parameters. Data have emerged ...

    • Unravelling the complexity of salt marsh ‘fucus cottonii’ forms (phaeophyceae, fucales) 

      Sjøtun, Kjersti; Heesch, Svenja; Rull Lluch, Jordi; Martín Martín, Rafael; Gómez Garreta, Amelia; Brysting, Anne K.; Coyer, James A. (Informa UK Limited, 2017-05-25)
      Genetic affiliation, nuclear DNA content, and gamete functioning were examined in small salt marsh Fucus from three localities in western Ireland. Individuals with small and dioecious receptacles were found at all localities, ...