Browsing by Author "Cwiklinski, Krystyna"
Now showing items 1-10 of 10
-
An atypical and functionally diverse family of Kunitz-type cysteine/serine proteinase inhibitors secreted by the helminth parasite Fasciola hepatica.
Smith, David; Cwiklinski, Krystyna; Jewhurst, Heather; Tikhonova, Irina G.; Dalton, John P. (Nature Research, 2020-11-26)Fasciola hepatica is a global parasite of humans and their livestock. Regulation of parasite-secreted cathepsin L-like cysteine proteases associated with virulence is important to fine-tune parasite-host interaction. We ... -
Biochemical and cellular characterisation of the Plasmodium falciparum M1 alanyl aminopeptidase (Pf M1AAP) and M17 leucyl aminopeptidase (Pf M17LAP)
Mathew, Rency; Wunderlich, Juliane; Thivierge, Karine; Cwiklinski, Krystyna; Dumont, Claire; Tilley, Leann; Rohrbach, Petra; Dalton, John P. (Nature Research, 2021-02-03)The Plasmodium falciparum M1 alanyl aminopeptidase and M17 leucyl aminopeptidase, PfM1AAP and PfM17LAP, are potential targets for novel anti-malarial drug development. Inhibitors of these aminopeptidases have been shown ... -
Complementary transcriptomic and proteomic analyses reveal the cellular and molecular processes that drive growth and development of Fasciola hepatica in the host liver
Cwiklinski, Krystyna; Robinson, Mark W.; Donnelly, Sheila; Dalton, John P. (BMC, 2021-01-11)Background The major pathogenesis associated with Fasciola hepatica infection results from the extensive tissue damage caused by the tunnelling and feeding activity of immature flukes during their migration, growth and ... -
Complex and dynamic transcriptional changes allow the helminth Fasciola gigantica to adjust to its intermediate snail and definitive mammalian hosts
Zhang, Xiao-Xuan; Cwiklinski, Krystyna; Hu, Rui-Si; Zheng, Wen-Bin; Sheng, Zhao-An; Zhang, Fu-Kai; Elsheikha, Hany M.; Dalton, John P.; Zhu, Xing-Quan (BMC (part of Springer Nature), 2019-10-12)The tropical liver fluke, Fasciola gigantica causes fasciolosis, an important disease of humans and livestock. We characterized dynamic transcriptional changes associated with the development of the parasite in its two ... -
Diagnosis of sheep fasciolosis caused by Fasciola hepatica using cathepsin L enzyme-linked immunosorbent assays (ELISA)
López Corrales, Jesús; Cwiklinski, Krystyna; De Marco Verissimo, Carolina; Dorey, Amber; Lalor, Richard; Jewhurst, Heather; McEvoy, Amanda; Diskin, Michael; Duffy, Catherine; Cosby, S Louise; Keane, Orla M; Dalton, John Pius (Elsevier, 2021-07-13)Fasciolosis, a global parasitic disease of agricultural livestock, is caused by the liver fluke Fasciola hepatica. Management and strategic control of fasciolosis on farms depends on early assessment of the extent of disease ... -
Fasciola hepatica serine protease inhibitor family (serpins): Purposely crafted for regulating host proteases
De Marco Verissimo, Carolina; Jewhurst, Heather L.; Tikhonova, Irina G.; Urbanus, Rolf T.; Maule, Aaron G.; Dalton, John P.; Cwiklinski, Krystyna (Public Library of Science, 2020-08-06)Serine protease inhibitors (serpins) regulate proteolytic events within diverse biological processes, including digestion, coagulation, inflammation and immune responses. The presence of serpins in Fasciola hepatica ... -
Fasciola hepaticasurface tegument: glycoproteins at the interface of parasite and host
Ravidà, Alessandra; Cwiklinski, Krystyna; Aldridge, Allison M.; Clarke, Paul; Thompson, Roisin; Gerlach, Jared Q.; Kilcoyne, Michelle; Hokke, Cornelis H.; Dalton, John P.; O'Neill, Sandra M. (American Society for Biochemistry & Molecular Biology (ASBMB), 2016-07-27)Fasciola hepatica, commonly known as liver fluke, is a trematode that causes Fasciolosis in ruminants and humans. The outer tegumental coat of F. hepatica (FhTeg) is a complex metabolically active biological matrix that ... -
Improved diagnosis of SARS-CoV-2 by using nucleoprotein and spike protein fragment 2 in quantitative dual ELISA tests
De Marco Verissimo, Carolina; O'Brien, Carol; López Corrales, Jesús; Dorey, Amber; Cwiklinski, Krystyna; Lalor, Richard; Doyle, Jack M.; Field, Stephen; Masterson, Claire; Ribes Martinez, Eduardo; Hughes, Gerry; Bergin, Colm; Walshe, Kieran; McNicholas, Bairbre; Laffey, John G.; Dalton, John P.; Kerr, Colm; Doyle, Sean (Cambridge University Press, 2021-06-08)The novel coronavirus, severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), is the causative agent of the 2020 worldwide coronavirus pandemic. Antibody testing is useful for diagnosing historic infections of a ... -
Schistosoma mansoni immunomodulatory molecule Sm16/SPO-1/SmSLP is a member of the trematode-specific helminth defence molecules (HDMs)
Shiels, Jenna; Cwiklinski, Krystyna; Alvarado, Raquel; Thivierge, Karine; Cotton, Sophie; Gonzales Santana, Bibiana; To, Joyce; Donnelly, Sheila; Taggart, Clifford C.; Weldon, Sinead; Dalton, John P. (Public Library of Science, 2020-07-09)Sm16, also known as SPO-1 and SmSLP, is a low molecular weight protein (~16kDa) secreted by the digenean trematode parasite Schistosoma mansoni, one of the main causative agents of human schistosomiasis. The molecule is ... -
Schistosoma mansoni immunomodulatory molecule Sm16/SPO-1/SmSLP is a member of the trematode-specific helminth defence molecules (HDMs)
Shiels, Jenna; Cwiklinski, Krystyna; Alvarado, Raquel; Thivierge, Karine; Cotton, Sophie; Gonzales Santana, Bibiana; To, Joyce; Donnelly, Sheila; Taggart, Clifford C.; Weldon, Sinead; Dalton, John P. (Public Library of Science, 2020-07-09)Background Sm16, also known as SPO-1 and SmSLP, is a low molecular weight protein (~16kDa) secreted by the digenean trematode parasite Schistosoma mansoni, one of the main causative agents of human schistosomiasis. The ...