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Functional expression of fasciola hepatica cathepsin l1 in saccharomyces cerevisiae
(Wiley-Blackwell, 1997-04-15)
A cDNA encoding the complete precursor of a Fasciola hepatica cathepsin L protease was isolated and sequenced. Functionally active enzyme was expressed and secreted by Saccharomyces cerevisiae transformed with a plasmid ...
Improved efficiency for primer extension by using a long, highly-labeled primer generated from immobilized single-stranded dna templates
(Oxford University Press (OUP), 1997-04-01)
Primer extension is one of the most common methods used to measure the amount and size of RNAs. We demonstrate that the sensitivity and the specificity of this method are improved considerably by using a highly-labeled ...