dc.contributor.author | Senchenkova, Sof'ya N. | |
dc.contributor.author | Shashkov, Alexander S. | |
dc.contributor.author | Moran, Antony P. | |
dc.contributor.author | Helander, Ilkka M. | |
dc.contributor.author | Knirel, Yuriy A. | |
dc.date.accessioned | 2018-08-24T08:26:22Z | |
dc.date.available | 2018-08-24T08:26:22Z | |
dc.date.issued | 1995-09-01 | |
dc.identifier.citation | Senchenkova, Sof'ya N. Shashkov, Alexander S.; Moran, Antony P.; Helander, Ilkka M.; Knirel, Yuriy A. (1995). Structures of the o-specific polysaccharide chains of pectinatus cerevisiiphilus and pectinatus frisingensis lipopolysaccharides. European Journal of Biochemistry 232 (2), 552-557 | |
dc.identifier.issn | 0014-2956,1432-1033 | |
dc.identifier.uri | http://hdl.handle.net/10379/9862 | |
dc.description.abstract | Mild acid hydrolysis of the smooth-type lipopolysaccharide (LPS) of Pectinatus frisingensis afforded no polysaccharide but monomeric 6-deoxy-L-altrose (L-6dAIt) which was identified by anion-exchange chromatography in berate buffer, GLC/MS, H-1-NMR spectroscopy, and optical rotation. LPS was degraded with alkali under reductive conditions to give a completely O-deacylated polysaccharide, which was studied by methylation analysis, H-1-NMR and C-13-NMR spectroscopy, including sequential, selective spin-decoupling, two-dimensional correlation spectroscopy (COSY), COSY with relayed coherence transfer, two-dimensional heteronuclear C-13, H-1-COSY, one-dimensional NOE and two-dimensional rotating-frame NOE spectroscopy. It was found that the O-specific polysaccharide chain of P. frisingensis LPS is a homopolymer of 6-deoxy-L-altrofuranose built up of tetrasaccharide-repeating units having the following structure:
[GRAPHICS]
Similarly, mild acid degradation of smooth-type LPS of Pectinatus cerevisiiphilus resulted in depolymerisation of the polysaccharide chain to give a disaccharide consisting of D-glucose and D-fucose, Study of the disaccharide by methylation analysis and alkali-degraded LPS by one-dimensional and two-dimensional H-1-NMR and C-13-NMR spectroscopy showed that the O-specific polysaccharide of P. cerevisiiphilus has the following structure:
-->2)-beta-D-Fucf-(1-->2)-alpha-D-Glcp-(1--> | |
dc.publisher | Wiley-Blackwell | |
dc.relation.ispartof | European Journal of Biochemistry | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | pectinatus | |
dc.subject | lipopolysaccharide | |
dc.subject | o-specific polysaccharide | |
dc.subject | structure | |
dc.subject | methylation | |
dc.subject | magnetic-resonance spectroscopy | |
dc.subject | pseudomonas-aeruginosa | |
dc.title | Structures of the o-specific polysaccharide chains of pectinatus cerevisiiphilus and pectinatus frisingensis lipopolysaccharides | |
dc.type | Article | |
dc.identifier.doi | 10.1111/j.1432-1033.1995.552zz.x | |
dc.local.publishedsource | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1995.552zz.x/pdf | |
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