The o-specific polysaccharide chain of campylobacter fetus serotype a lipopolysaccharide is a partially o-acetylated 1,3-linked alpha-d-mannan
Senchenkova, Sof'ya N.
Shashkov, Alexander S.
Knirel, Yuny A.
Mcgovern, Joseph J.
Moran, Anthony P.
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Senchenkova, Sof'ya N. Shashkov, Alexander S.; Knirel, Yuny A.; Mcgovern, Joseph J.; Moran, Anthony P. (1997). The o-specific polysaccharide chain of campylobacter fetus serotype a lipopolysaccharide is a partially o-acetylated 1,3-linked alpha-d-mannan. European Journal of Biochemistry 245 (3), 637-641
A polysaccharide fraction liberated from Campylobacter fetus subsp. fetus serotype A lipopolysaccharide by mild acid hydrolysis followed by gel-permeation chromatography contained a partially O-acetylated D-mannan chain, as an O-specific polysaccharide, with a core oligosaccharide attached. The structure of the polysaccharide was studied by O-deacetylation, methylation, and H-1- and C-13-NMR spectroscopy, including computer-assisted analysis of the C-13-NMR spectrum. A structure of -->3)-alpha-D-Manp2Ac-(1--> was established as the structure of the O-specific polysaccharide, the degree of O-acetylation of the mannose residues at position 2 being estimated as 80-90%. As judged by the ratio of mannose to core constituents, the D-mannan chain consists on average of 10-12 monosaccharide units.