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dc.contributor.authorNilsson, C.
dc.contributor.authorSkoglund, A.
dc.contributor.authorMoran, A. P.
dc.contributor.authorAnnuk, H.
dc.contributor.authorEngstrand, L.
dc.contributor.authorNormark, S.
dc.identifier.citationNilsson, C. Skoglund, A.; Moran, A. P.; Annuk, H.; Engstrand, L.; Normark, S. (2006). An enzymatic ruler modulates lewis antigen glycosylation of helicobacter pylori lps during persistent infection. Proceedings of the National Academy of Sciences 103 (8), 2863-2868
dc.description.abstractHelicobacter pylori persistently colonizes about half the human population and contributes to the development of peptic ulcer disease and gastric cancer. This organism has evolved means to structurally alter its surface characteristics to evade innate and adaptive immune responses. H. pylori produces LIPS O-antigen units that can be posttranslationally fucosylated to generate Lewis antigens, structures also found on human epithelial cells. We demonstrate an extensive diversity of Lewis x and Lewis y expression in LPS O-antigen units, occurring over time and in different regions of the human stomach. Lewis expression patterns were correlated with the on/off status of the three fucosyltransferases (FucT), FutA, FutB, and FutC, which are regulated via slipped-strand mispairing in intragenic polyC tract regions of the corresponding genes. The alpha 1,3-FucT, FutA and FutB, each contain a C-terminal heptad repeat region, consisting of a variable number of DD/NLRV/INY tandem repeats. Variations in the number of heptad repeats correlated to the sizes of O-antigen polymers to become decorated by fucose residues. Our data support a molecular ruler mechanism for how H. pylori varies its LPS fucosylation pattern, where one heptad repeat in the enzyme corresponds to one N-acetyl-beta-lactosamine unit in the O-antigen polysaccharide.
dc.publisherProceedings of the National Academy of Sciences
dc.relation.ispartofProceedings of the National Academy of Sciences
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.subjecthuman stomach
dc.subjectphase variation
dc.subjectcore oligosaccharide regions
dc.subjectblood-group antigens
dc.subjectphase variation
dc.subjectmolecular mimicry
dc.subjecto antigen
dc.titleAn enzymatic ruler modulates lewis antigen glycosylation of helicobacter pylori lps during persistent infection

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