The impact of differential expression of extracellular matrix metalloproteinase inducer, matrix metalloproteinase-2, tissue inhibitor of matrix metalloproteinase-2 and pdgf-aa on the chronicity of venous leg ulcers
MetadataShow full item record
This item's downloads: 0 (view details)
Mwaura, B. Mahendran, B.; Hynes, N.; Defreitas, D.; Avalos, G.; Adegbola, T.; Adham, M.; Connolly, C.E.; Sultan, S. (2006). The impact of differential expression of extracellular matrix metalloproteinase inducer, matrix metalloproteinase-2, tissue inhibitor of matrix metalloproteinase-2 and pdgf-aa on the chronicity of venous leg ulcers. European Journal of Vascular and Endovascular Surgery 31 (3), 306-310
Introduction. Alteration in the expression of extracellular matrix metalloproteinase inducer (EMMPRIN), matrix metalloproteinase-2 (MMP-2), tissue inhibitors of matrix metalloproteinases (TIMP-2) and platelet derived growth factor (PDGF-AA) may contribute to poor healing in venous leg ulcers. Aim. The aim of this study is to determine the expression of EMMPRIN, MMP-2, TIMP-2 and PDGF-AA in the ulcer exudates and perivascular tissue of healing and non-healing chronic venous ulcers. Patients, materials and methods. Forty patients with chronic venous ulcers were included in this study, with a mean age of 60 years. Eleven patients were males and 29 were females. All patients had normal ankle brachial index and a venous ulcer of at least 8 weeks duration. Immuno-histochemistry using monoclonal antibodies to PDGF-AA, MMP-2, TIMP-2 and EMMPRIN was carried out on paraffin embedded punch biopsy skin specimens from the ulcer edge. Enzyme linked immunosorbent assay for PDGF, MMP2 and TIMP-2 were carried out on wound fluids collected from patients. The ulcer size and character at the initial assessment and after 8 weeks were assessed to determine the status of ulcer healing. Results. No significant difference was seen in the expression of TIMP-2, MMP-2 and EMMPRIN between the two groups. However, in the non-healing group high levels of MMP-2 and low levels of TIMP-2 in the wound fluid suggest a strong correlation of these two markers in the state of healing. Analysis of wound fluid by ELISA demonstrated high PDGF-AA in the healing group (p = 0.021). Significantly increased levels of PDGF-AA (p < 0001) was noted in the perivascular area on immuno-histochemistry of healing ulcers. These data suggest that PDGF-AA plays an important role in healing of venous ulcers. Conclusion. Non-healing venous ulcers are associated with greater activity MMP-2 activity. The ratio of MMPs to their inhibitors TIMPs, dictate the rate of healing of the ulcers. PDGF-AA activity is associated with ulcer healing, though the mechanism is unclear. EMMPRIN expression in chronic venous ulcers probably parallels the chronicity of the condition rather than propagate it. However, further studies with larger samples are needed.
Showing items related by title, author, creator and subject.
Macromolecular crowding meets tissue engineering by self-ssembly: A paradigm shift in regenerative medicine Satyam, Abhigyan; Kumar, Pramod; Fan, Xingliang; Gorelov, Alexander; Rochev, Yury; Joshi, Lokesh; Peinado, Héctor; Lyden, David; Thomas, Benjamin; Rodriguez, Brian; Raghunath, Michael; Pandit, Abhay; Zeugolis, Dimitrios (Wiley, 2014-02-06)Advancements in molecular and cell biology have led to the development of cell-based therapies to treat injured or degenerated tissues.  The rationale of this concept is that functional regeneration can be achieved ...
Differential expression of the metalloproteinase MMP3 and the a5 integrin subunit in human myometrium at labour Ahamide, Elodie; Morrison, John J.; O Shaughnessy, Deirdre; O Brien, Margaret; Smith, Terry (Oxford University Press, 2007-09-13)Extensive tissue remodelling occurs in the human myometrium before, during and after parturition. The aim of this study was to investigate the expression of two tissue remodelling molecules, matrix metalloproteinase 3 ...
Sar150640, a selective β3-adrenoceptor agonist, prevents human myometrial remodelling and activation of matrix metalloproteinase in an in vitro model of chorioamnionitis Lirussi, F; O'Brien, M; Wendremaire, M; Goirand, F; Sagot, P; Dumas, M; Morrison, JJ; Bardou, M (Wiley-Blackwell, 2010-02-05)Background and purpose: The uterine pathophysiology underlying inflammatory conditions such as chorioamnionitis remains largely unclear. As we have shown that beta(3)-adrenoceptors act as regulators of myometrial inflammation, ...