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dc.contributor.authorClancy, Cillian
dc.contributor.authorKhan, Sonja
dc.contributor.authorGlynn, Claire L.
dc.contributor.authorHolian, Emma
dc.contributor.authorDockery, Peter
dc.contributor.authorLalor, Pierce
dc.contributor.authorBrown, James A.L.
dc.contributor.authorJoyce, Myles R.
dc.contributor.authorKerin, Michael J.
dc.contributor.authorDwyer, Róisín M.
dc.date.accessioned2019-09-04T09:00:25Z
dc.date.available2019-09-04T09:00:25Z
dc.date.issued2017-01-03
dc.identifier.citationClancy, C,Khan, S,Glynn, CL,Holian, E,Dockery, P,Lalor, P,Brown, JAL,Joyce, MR,Kerin, MJ,Dwyer, RM (2016) 'Screening of exosomal microRNAs from colorectal cancer cells'. Cancer Biomarkers, 17 :427-435, DOI: 10.3233/CBM-160659en_IE
dc.identifier.issn1875-8592
dc.identifier.urihttp://hdl.handle.net/10379/15382
dc.description.abstractBACKGROUND: Cells release extracellular membrane vesicles including microvesicles known as exosomes. Exosomes contain microRNAs (miRNAs) however the full range within colorectal cancer cell secreted exosomes is unknown.OBJECTIVE: To identify the full range of exosome encapsulated miRNAs secreted from 2 colorectal cancer cell lines and to investigate engineering of exosomes over-expressing miRNAs.METHODS: Exosomes were isolated from HCT-116 and HT-29 cell lines. RNA was extracted from exosomes and microRNA array performed. Cells were engineered to express miR-379 (HCT-116-379) or a non-targeting control (HCT-116-NTC) and functional effects were determined. Exosomes secreted by engineered cells were transferred to recipient cells and the impact examined.RESULTS: Microvesicles 40-100 nm in size secreted by cell lines were visualised and confirmed to express exosomal protein CD63. HT-29 exosomes contained 409 miRNAs, HCT-116 exosomes contained 393, and 338 were common to exosomes from both cell lines. Selected targets were validated. HCT-116-379 cells showed decreased proliferation (12-15% decrease, pen_IE
dc.description.sponsorshipFunding support was received from Breast Cancer Research and the Irish Cancer Society Collaborative Cancer Research Centre BREAST-PREDICT Grant CCRC13GAL.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherIOS Pressen_IE
dc.relation.ispartofCancer Biomarkersen
dc.subjectExosomesen_IE
dc.subjectmicroRNAsen_IE
dc.subjectcolorectal canceren_IE
dc.subjectBREAST-CANCERen_IE
dc.subjectMEDIATED TRANSFERen_IE
dc.subjectMESSENGER-RNASen_IE
dc.subjectEXPRESSIONen_IE
dc.subjectINVASIONen_IE
dc.subjectMIR-378en_IE
dc.titleScreening of exosomal microRNAs from colorectal cancer cellsen_IE
dc.typeArticleen_IE
dc.date.updated2019-08-16T10:12:30Z
dc.identifier.doi10.3233/CBM-160659
dc.local.publishedsourcehttps://dx.doi.org/10.3233/CBM-160659en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderBreast Cancer Researchen_IE
dc.contributor.funderIrish Cancer Societyen_IE
dc.internal.rssid12198686
dc.local.contactRóisín Dwyer, Surgery, Nui Galway. 3008 Email: roisin.dwyer@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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