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dc.contributor.authorOkine, Bright N.
dc.contributor.authorGaspar, Jessica C.
dc.contributor.authorMadasu, Manish K.
dc.contributor.authorOlango, Weredeselam M.
dc.contributor.authorHarhen, Brendan
dc.contributor.authorRoche, Michelle
dc.contributor.authorFinn, David P.
dc.date.accessioned2019-03-29T13:50:56Z
dc.date.available2019-03-29T13:50:56Z
dc.date.issued2016-12-01
dc.identifier.citationOkine, Bright N., Gaspar, Jessica C., Madasu, Manish K., Olango, Weredeselam M., Harhen, Brendan, Roche, Michelle, & Finn, David P. (2017). Characterisation of peroxisome proliferator-activated receptor signalling in the midbrain periaqueductal grey of rats genetically prone to heightened stress, negative affect and hyperalgesia. Brain Research, 1657, 185-192. doi: https://doi.org/10.1016/j.brainres.2016.11.022en_IE
dc.identifier.issn1872-6240
dc.identifier.urihttp://hdl.handle.net/10379/15076
dc.description.abstractThe stress-hyperresponsive Wistar-Kyoto (WRY) rat strain exhibits a hyperalgesic phenotype and is a useful genetic model for studying stress-pain interactions. Peroxisome proliferator-activated receptor (PPAR) signalling in the midbrain periaqueductal grey (PAG) modulates pain. This study characterised PPAR signalling in the PAG of WRY rats exposed to the formalin test of inflammatory pain, versus Sprague-Dawley (SD) controls.Formalin injection reduced levels of the endogenous PPAR ligands N-palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA) in the lateral(1) PAG of SD rats, but not WRY rats which exhibited higher levels of these analytes compared with formalin-injected SD counterparts. Levels of mRNA coding for fatty acid amide hydrolase (FAAH; catabolises PEA and OEA) were lower in the]PAG of WRY versus SD rats. PPARy mRNA and protein levels in the IPAG were higher in saline-treated WRY rats, with PPARy protein levels reduced by formalin treatment in WRY rats only. In the dorsolateral(dl) or ventrolateral(v1) PAG, there were no effects of formalin injection on PEA or OEA levels but there were some differences in levels of these analytes between saline-treated WRY and SD rats and some formalin-evoked alterations in levels of PPARy, PPARy or FAAH mRNA in WKY and/or SD rats. Pharmacological blockade of PPARy in the IPAG enhanced formalin-evoked nociceptive behaviour in WRY, but not SD, rats.These data indicate differences in the PPAR signalling system in the PAG of WRY versus SD rats and suggest that enhanced PEA/OEA-mediated tone at PPARy in the IPAG may represent an adaptive mechanism to lower hyperalgesia in WRY rats. (C) 2016 Elsevier B.V. All rights reserved.en_IE
dc.description.sponsorshipThis work was funded by grants from Science Foundation Ireland (10/IN.1/B2976), The Irish Research Council, CNPq - Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil (207530/2014-9) and the National University of Ireland Galway.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherElsevieren_IE
dc.relation.ispartofBrain Researchen
dc.subjectWistar-Kyoto (WKY)en_IE
dc.subjectPeroxisome proliferator-activated receptor (PPAR)en_IE
dc.subjectDescending pain pathwayen_IE
dc.subjectFormalinen_IE
dc.subjectN-palmitoylethanolamide (PEA)en_IE
dc.subjectN-oleoylethanolamide (OEA)en_IE
dc.subjectFATTY-ACID AMIDEen_IE
dc.subjectSTIMULATION-PRODUCED ANALGESIAen_IE
dc.subjectWISTAR-KYOTO RATen_IE
dc.subjectPPAR-ALPHAen_IE
dc.subjectNEUROPATHIC PAINen_IE
dc.subjectFORMALIN TESTen_IE
dc.subjectPALMITOYLETHANOLAMIDEen_IE
dc.subjectINHIBITIONen_IE
dc.subjectMODELen_IE
dc.subjectDEPRESSIONen_IE
dc.titleCharacterisation of peroxisome proliferator-activated receptor signalling in the midbrain periaqueductal grey of rats genetically prone to heightened stress, negative affect and hyperalgesiaen_IE
dc.typeArticleen_IE
dc.date.updated2019-03-27T13:54:53Z
dc.identifier.doi10.1016/j.brainres.2016.11.022
dc.local.publishedsourcehttps://doi.org/10.1016/j.brainres.2016.11.022en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderScience Foundation Irelanden_IE
dc.contributor.funderIrish Research Councilen_IE
dc.contributor.funderConselho Nacional de Desenvolvimento Científico e Tecnológicoen_IE
dc.contributor.funderNational University of Ireland, Galwayen_IE
dc.internal.rssid12329154
dc.local.contactDavid Finn, Dept. Of Pharmacology &, Therapeutics, Nui, Galway. 5280 Email: david.finn@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
dcterms.projectinfo:eu-repo/grantAgreement/SFI/SFI Principal Investigator Programme (PI)/10/IN.1/B2976/IE/The role of the endocannabinoid system in anxiety-induced modulation of pain: sites and mechanisms of action/en_IE
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