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dc.contributor.authorSiddaiah, Chandra Nayaka
dc.contributor.authorSatyanarayana, Niranjan Raj
dc.contributor.authorMudili, Venkataramana
dc.contributor.authorKumar Gupta, Vijai
dc.contributor.authorGurunathan, Selvakumar
dc.contributor.authorRangappa, Shobith
dc.contributor.authorHuntrike, Shekar Shetty
dc.contributor.authorSrivastava, Rakesh Kumar
dc.date.accessioned2018-09-20T16:24:40Z
dc.date.available2018-09-20T16:24:40Z
dc.date.issued2017-03-21
dc.identifier.citationSiddaiah, Chandra Nayaka; Satyanarayana, Niranjan Raj; Mudili, Venkataramana; Kumar Gupta, Vijai; Gurunathan, Selvakumar; Rangappa, Shobith; Huntrike, Shekar Shetty; Srivastava, Rakesh Kumar (2017). Elicitation of resistance and associated defense responses in trichoderma hamatum induced protection against pearl millet downy mildew pathogen. Scientific Reports 7 ,
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10379/13904
dc.description.abstractEndophytic Trichoderma hamatum UoM 13 isolated from pearl millet roots was evaluated for its efficiency to suppress downy mildew disease. Under laboratory conditions, T. hamatum seed treatment significantly enhanced pearl millet seed germination and seedling vigor. T. hamatum seed treatment resulted in systemic and durable immunity against pearl millet downy mildew disease under greenhouse and field conditions. T. hamatum treated seedlings responded to downy mildew infection with high lignification and callose deposition. Analysis of defense enzymes showed that T. hamatum treatment significantly enhanced the activities of glucanase, peroxidase, phenylalanine ammonia-lyase, and polyphenol oxidase in comparison to untreated control. RT-PCR analysis revealed differentially expressed transcripts of the defense enzymes and PR-proteins in treated, untreated, and checks, wherein PR-1, PR-5, and cell wall defense HRGPs were significantly over expressed in treated seedlings as against their lower expression in controls. T. hamatum treatment significantly stimulated endogenous salicylic acid ( SA) levels and significantly upregulated important SA biosynthesis gene isochorismate synthase. The results indicated that T. hamatum UoM13 treatment induces resistance corresponding to significant over expression of endogenous SA, important defense enzymes, PR-proteins, and HRGPs, suggesting that SA biosynthetic pathway is involved in pearl millet for mounting systemic immunity against downy mildew pathogen.
dc.publisherSpringer Nature
dc.relation.ispartofScientific Reports
dc.subjectinduced systemic resistance
dc.subjectsalicylic-acid
dc.subjectarabidopsis-thaliana
dc.subjectcell-wall
dc.subjectpseudomonas-syringae
dc.subjectrhizoctonia-solani
dc.subjectdisease resistance
dc.subjectpolyphenol oxidase
dc.subjectgene-expression
dc.subjectharzianum t39
dc.titleElicitation of resistance and associated defense responses in trichoderma hamatum induced protection against pearl millet downy mildew pathogen
dc.typeArticle
dc.identifier.doi10.1038/srep43991
dc.local.publishedsourcehttp://www.nature.com/articles/srep43991.pdf
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