dc.contributor.author | Robertson, Ruairi | |
dc.contributor.author | Guihéneuf, Freddy | |
dc.contributor.author | Bahar, Bojlul | |
dc.contributor.author | Schmid, Matthias | |
dc.contributor.author | Stengel, Dagmar | |
dc.contributor.author | Fitzgerald, Gerald | |
dc.contributor.author | Ross, R. | |
dc.contributor.author | Stanton, Catherine | |
dc.date.accessioned | 2018-09-20T16:23:00Z | |
dc.date.available | 2018-09-20T16:23:00Z | |
dc.date.issued | 2015-08-20 | |
dc.identifier.citation | Robertson, Ruairi; Guihéneuf, Freddy; Bahar, Bojlul; Schmid, Matthias; Stengel, Dagmar; Fitzgerald, Gerald; Ross, R. Stanton, Catherine (2015). The anti-inflammatory effect of algae-derived lipid extracts on lipopolysaccharide (lps)-stimulated human thp-1 macrophages. Marine Drugs 13 (8), 5402-5424 | |
dc.identifier.issn | 1660-3397 | |
dc.identifier.uri | http://hdl.handle.net/10379/13673 | |
dc.description.abstract | Algae contain a number of anti-inflammatory bioactive compounds such as omega-3 polyunsaturated fatty acids (n-3 PUFA) and chlorophyll a, hence as dietary ingredients, their extracts may be effective in chronic inflammation-linked metabolic diseases such as cardiovascular disease. In this study, anti-inflammatory potential of lipid extracts from three red seaweeds (Porphyra dioica, Palmaria palmata and Chondrus crispus) and one microalga (Pavlova lutheri) were assessed in lipopolysaccharide (LPS)-stimulated human THP-1 macrophages. Extracts contained 34%-42% total fatty acids as n-3 PUFA and 5%-7% crude extract as pigments, including chlorophyll a, -carotene and fucoxanthin. Pretreatment of the THP-1 cells with lipid extract from P. palmata inhibited production of the pro-inflammatory cytokines interleukin (IL)-6 (p < 0.05) and IL-8 (p < 0.05) while that of P. lutheri inhibited IL-6 (p < 0.01) production. Quantitative gene expression analysis of a panel of 92 genes linked to inflammatory signaling pathway revealed down-regulation of the expression of 14 pro-inflammatory genes (TLR1, TLR2, TLR4, TLR8, TRAF5, TRAF6, TNFSF18, IL6R, IL23, CCR1, CCR4, CCL17, STAT3, MAP3K1) by the lipid extracts. The lipid extracts effectively inhibited the LPS-induced pro-inflammatory signaling pathways mediated via toll-like receptors, chemokines and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-B) signaling molecules. These results suggest that lipid extracts from P. lutheri, P. palmata, P. dioica and C. crispus can inhibit LPS-induced inflammatory pathways in human macrophages. Therefore, algal lipid extracts should be further explored as anti-inflammatory ingredients for chronic inflammation-linked metabolic diseases. | |
dc.publisher | MDPI AG | |
dc.relation.ispartof | Marine Drugs | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | microalgae | |
dc.subject | macroalgae | |
dc.subject | thp-1 | |
dc.subject | inflammation | |
dc.subject | lipids | |
dc.subject | n-3 pufa | |
dc.subject | polyunsaturated fatty acids | |
dc.subject | macrophages | |
dc.subject | chlorophyll a | |
dc.subject | bioactive pigments | |
dc.subject | polyunsaturated fatty-acids | |
dc.subject | pro-inflammatory mediators | |
dc.subject | raw 264.7 macrophages | |
dc.subject | nf-kappa-b | |
dc.subject | pavlova-lutheri | |
dc.subject | cardiovascular-disease | |
dc.subject | docosahexaenoic acid | |
dc.subject | metabolic syndrome | |
dc.subject | arachidonic-acid | |
dc.subject | gene-expression | |
dc.title | The anti-inflammatory effect of algae-derived lipid extracts on lipopolysaccharide (lps)-stimulated human thp-1 macrophages | |
dc.type | Article | |
dc.identifier.doi | 10.3390/md13085402 | |
dc.local.publishedsource | http://www.mdpi.com/1660-3397/13/8/5402/pdf | |
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