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dc.contributor.authorPielach, Anna
dc.contributor.authorLeroux, Olivier
dc.contributor.authorDomozych, David S.
dc.contributor.authorKnox, J. Paul
dc.contributor.authorPopper, Zoë A.
dc.identifier.citationPielach, Anna; Leroux, Olivier; Domozych, David S. Knox, J. Paul; Popper, Zoë A. (2014). Arabinogalactan protein-rich cell walls, paramural deposits and ergastic globules define the hyaline bodies of rhinanthoid orobanchaceae haustoria. Annals of Botany 114 (6), 1359-1373
dc.description.abstractBackground and Aims Parasitic plants obtain nutrients from their hosts through organs called haustoria. The hyaline body is a specialized parenchymatous tissue occupying the central parts of haustoria in many Orobanchaceae species. The structure and functions of hyaline bodies are poorly understood despite their apparent necessity for the proper functioning of haustoria. Reported here is a cell wall-focused immunohistochemical study of the hyaline bodies of three species from the ecologically important clade of rhinanthoid Orobanchaceae. Methods Haustoria collected from laboratory-grown and field-collected plants of Rhinanthus minor, Odontites vernus and Melampyrum pratense attached to various hosts were immunolabelled for cell wall matrix glycans and glycoproteins using specific monoclonal antibodies (mAbs). Key Results Hyaline body cell wall architecture differed from that of the surrounding parenchyma in all species investigated. Enrichment in arabinogalactan protein (AGP) epitopes labelled with mAbs LM2, JIM8, JIM13, JIM14 and CCRC-M7 was prominent and coincided with reduced labelling of de-esterified homogalacturonan with mAbs JIM5, LM18 and LM19. Furthermore, paramural bodies, intercellular deposits and globular ergastic bodies composed of pectins, xyloglucans, extensins and AGPs were common. In Rhinanthus they were particularly abundant in pairings with legume hosts. Hyaline body cells were not in direct contact with haustorial xylem, which was surrounded by a single layer of paratracheal parenchyma with thickened cell walls abutting the xylem. Conclusions The distinctive anatomy and cell wall architecture indicate hyaline body specialization. Altered proportions of AGPs and pectins may affect the mechanical properties of hyaline body cell walls. This and the association with a transfer-like type of paratracheal parenchyma suggest a role in nutrient translocation. Organelle-rich protoplasts and the presence of exceptionally profuse intra-and intercellular wall materials when attached to a nitrogen-fixing host suggest subsequent processing and transient storage of nutrients. AGPs might therefore be implicated in nutrient transfer and metabolism in haustoria.
dc.publisherOxford University Press (OUP)
dc.relation.ispartofAnnals of Botany
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.subjecthyaline body
dc.subjectarabinogalactan proteins
dc.subjectrhinanthoid orobanchaceae
dc.subjectcell wall
dc.subjectparasitic plant
dc.subjectparamural body
dc.subjectglobular ergastic body
dc.subjectrhinanthus minor
dc.subjectodontites vernus
dc.subjectmelampyrum pratense
dc.subjectpectic homogalacturonan
dc.subjectparasitic plants
dc.titleArabinogalactan protein-rich cell walls, paramural deposits and ergastic globules define the hyaline bodies of rhinanthoid orobanchaceae haustoria

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