Sensitive surface enhanced raman scattering multiplexed detection of matrix metalloproteinase 2 and 7 cancer markers
Kong, Kien Voon
MetadataShow full item record
This item's downloads: 0 (view details)
Gong, Tianxun; Kong, Kien Voon; Goh, Douglas; Olivo, Malini; Yong, Ken-Tye (2015). Sensitive surface enhanced raman scattering multiplexed detection of matrix metalloproteinase 2 and 7 cancer markers. Biomedical Optics Express 6 (6), 2076-2087
A surface enhanced Raman spectroscopy (SERS) based platform was developed for sensitive multiplexed detection of matrix metalloproteinases (MMP) (MMP-2 and MMP-7) with low limit of detection and high specificity. Detection is based on the virtue of enzymatic reaction where a peptide can be cleaved only by its corresponding enzyme. The platform comprises two components, a specialized SERS-based bimetallic-film-over-nanosphere (BMFON) substrate and gold nanoparticles (AuNPs). The two components were functionalized such that binding between the two would occur through biotin-avidin-biotin complexation. Binding is hindered by MMP peptide chains conjugated onto the surfaces of the substrate and AuNPs, and can be removed only by cleaving the peptide chains with corresponding enzymes. Since AuNP binding sites become free after the peptides are cleaved, the number of binding sites for AuNPs onto the substrate would increase. By tagging the AuNPs, concentrations of MMP-specific enzymes can be quantified through examining intensities of signature SERS peaks of the tags. This cleave-and-bind mechanism was first validated by individual detection and quantification of MMP-2 and MMP-7. The platform was demonstrated to be able to sensitively detect concentrations of specific enzymes ranging from 1 ng/mL to 40 mu g/mL, with close correlation between SERS intensity and concentrations. Finally, the multiplexed detection of MMP-2 and MMP-7 was demonstrated. The multiplexity of this platform provides a robust way to analyze diseases associated with MMP-2 and MMP-7 enzymes. Our work can be further developed as a clinical diagnostic tool to detect other MMP proteinase in bio-fluids samples, widening the number of biomarkers needed to characterize diseases better. (C)2015 Optical Society of America
Showing items related by title, author, creator and subject.
Mcinerney, Niall; Colleran, Gabrielle; Rowan, Andrew; Walther, Axel; Barclay, Ella; Spain, Sarah; Jones, Angela M.; Tuohy, Stephen; Curran, Catherine; Miller, Nicola; Kerin, Michael; Tomlinson, Ian; Sawyer, Elinor (Springer Nature, 2008-11-13)Large scale association studies have identified low penetrance susceptibility alleles that predispose to breast cancer. A locus on chromosome 8q24.21 has been shown to harbour variants that predispose to breast, ovarian, ...
Systemic mirna-195 differentiates breast cancer from other malignancies and is a potential biomarker for detecting noninvasive and early stage disease Heneghan, H. M.; Miller, N.; Kelly, R.; Newell, J.; Kerin, M. J. (Alphamed Press, 2010-06-24)Purpose. The potential of microRNAs (miRNAs) as novel tumor markers has been the focus of recent scrutiny because of their tissue specificity, stability, and association with clinicopathological parameters. Data have emerged ...
McAnena, Peter; Brown, James; Kerin, Michael (MDPI AG, 2017-01-08)Traditionally the stratification of many cancers involves combining tumour and clinicopathological features (e.g., patient age; tumour size, grade, receptor status and location) to inform treatment options and predict ...