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dc.contributor.authorFarrell, Eric
dc.contributor.authorFahy, Niamh
dc.contributor.authorRyan, Aideen E
dc.contributor.authorFlatharta, Cathal O
dc.contributor.authorO’Flynn, Lisa
dc.contributor.authorRitter, Thomas
dc.contributor.authorMurphy, J Mary
dc.date.accessioned2018-09-20T16:07:41Z
dc.date.available2018-09-20T16:07:41Z
dc.date.issued2016-05-18
dc.identifier.citationFarrell, Eric; Fahy, Niamh; Ryan, Aideen E; Flatharta, Cathal O; O’Flynn, Lisa; Ritter, Thomas; Murphy, J Mary (2016). Vil-10-overexpressing human mscs modulate naïve and activated t lymphocytes following induction of collagenase-induced osteoarthritis. Stem Cell Research & Therapy 7 ,
dc.identifier.issn1757-6512
dc.identifier.urihttp://hdl.handle.net/10379/11416
dc.description.abstractBackground: Recent efforts in osteoarthritis (OA) research have highlighted synovial inflammation and involvement of immune cells in disease onset and progression. We sought to establish the in-vivo immune response in collagenase-induced OA and investigate the ability of human mesenchymal stem cells (hMSCs) overexpressing viral interleukin 10 (vIL-10) to modulate immune populations and delay/prevent disease progression. Methods: Eight-week-old male C57BL/6 mice were injected with 1 U type VII collagenase over two consecutive days. At day 7, 20,000 hMSCs overexpressing vIL-10 were injected into the affected knee. Control groups comprised of vehicle, 20,000 untransduced or adNull-transduced MSCs or virus alone. Six weeks later knees were harvested for histological analysis and popliteal and inguinal lymph nodes for flow cytometric analysis. Results: At this time there was no significant difference in knee OA scores between any of the groups. A trend toward more damage in animals treated with hMSCs was observed. Interestingly there was a significant reduction in the amount of activated CD4 and CD8 T cells in the vIL-10-expressing hMSC group. Conclusions: vIL-10-overexpressing hMSCs can induce long-term reduction in activated T cells in draining lymph nodes of mice with collagenase-induced OA. This could lead to reduced OA severity or disease progression over the long term.
dc.publisherSpringer Nature
dc.relation.ispartofStem Cell Research & Therapy
dc.subjectcollagenase-induced osteoarthritis
dc.subjectmesenchymal stem cell
dc.subjectvil-10
dc.subjectcell therapy
dc.subjectgene therapy
dc.subjectxenogeneic
dc.subjectmesenchymal stem-cells
dc.subjectinduced arthritis
dc.subjectin-vitro
dc.subjectintraarticular injection
dc.subjectstimulatory factor
dc.subjectimmune-response
dc.subjectb-cells
dc.subjectinterleukin-10
dc.subjectcartilage
dc.subjectdifferentiation
dc.titleVil-10-overexpressing human mscs modulate naïve and activated t lymphocytes following induction of collagenase-induced osteoarthritis
dc.typeArticle
dc.identifier.doi10.1186/s13287-016-0331-2
dc.local.publishedsourcehttps://stemcellres.biomedcentral.com/track/pdf/10.1186/s13287-016-0331-2?site=stemcellres.biomedcentral.com
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