dc.contributor.author | Farrell, Eric | |
dc.contributor.author | Fahy, Niamh | |
dc.contributor.author | Ryan, Aideen E | |
dc.contributor.author | Flatharta, Cathal O | |
dc.contributor.author | O’Flynn, Lisa | |
dc.contributor.author | Ritter, Thomas | |
dc.contributor.author | Murphy, J Mary | |
dc.date.accessioned | 2018-09-20T16:07:41Z | |
dc.date.available | 2018-09-20T16:07:41Z | |
dc.date.issued | 2016-05-18 | |
dc.identifier.citation | Farrell, Eric; Fahy, Niamh; Ryan, Aideen E; Flatharta, Cathal O; O’Flynn, Lisa; Ritter, Thomas; Murphy, J Mary (2016). Vil-10-overexpressing human mscs modulate naïve and activated t lymphocytes following induction of collagenase-induced osteoarthritis. Stem Cell Research & Therapy 7 , | |
dc.identifier.issn | 1757-6512 | |
dc.identifier.uri | http://hdl.handle.net/10379/11416 | |
dc.description.abstract | Background: Recent efforts in osteoarthritis (OA) research have highlighted synovial inflammation and involvement of immune cells in disease onset and progression. We sought to establish the in-vivo immune response in collagenase-induced OA and investigate the ability of human mesenchymal stem cells (hMSCs) overexpressing viral interleukin 10 (vIL-10) to modulate immune populations and delay/prevent disease progression.
Methods: Eight-week-old male C57BL/6 mice were injected with 1 U type VII collagenase over two consecutive days. At day 7, 20,000 hMSCs overexpressing vIL-10 were injected into the affected knee. Control groups comprised of vehicle, 20,000 untransduced or adNull-transduced MSCs or virus alone. Six weeks later knees were harvested for histological analysis and popliteal and inguinal lymph nodes for flow cytometric analysis.
Results: At this time there was no significant difference in knee OA scores between any of the groups. A trend toward more damage in animals treated with hMSCs was observed. Interestingly there was a significant reduction in the amount of activated CD4 and CD8 T cells in the vIL-10-expressing hMSC group.
Conclusions: vIL-10-overexpressing hMSCs can induce long-term reduction in activated T cells in draining lymph nodes of mice with collagenase-induced OA. This could lead to reduced OA severity or disease progression over the long term. | |
dc.publisher | Springer Nature | |
dc.relation.ispartof | Stem Cell Research & Therapy | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | collagenase-induced osteoarthritis | |
dc.subject | mesenchymal stem cell | |
dc.subject | vil-10 | |
dc.subject | cell therapy | |
dc.subject | gene therapy | |
dc.subject | xenogeneic | |
dc.subject | mesenchymal stem-cells | |
dc.subject | induced arthritis | |
dc.subject | in-vitro | |
dc.subject | intraarticular injection | |
dc.subject | stimulatory factor | |
dc.subject | immune-response | |
dc.subject | b-cells | |
dc.subject | interleukin-10 | |
dc.subject | cartilage | |
dc.subject | differentiation | |
dc.title | Vil-10-overexpressing human mscs modulate naïve and activated t lymphocytes following induction of collagenase-induced osteoarthritis | |
dc.type | Article | |
dc.identifier.doi | 10.1186/s13287-016-0331-2 | |
dc.local.publishedsource | https://stemcellres.biomedcentral.com/track/pdf/10.1186/s13287-016-0331-2?site=stemcellres.biomedcentral.com | |
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