dc.contributor.author | Dufour, Florent | |
dc.contributor.author | Rattier, Thibault | |
dc.contributor.author | Shirley, Sarah | |
dc.contributor.author | Picarda, Gaelle | |
dc.contributor.author | Constantinescu, Andrei Alexandru | |
dc.contributor.author | Morlé, Aymeric | |
dc.contributor.author | Zakaria, Al Batoul | |
dc.contributor.author | Marcion, Guillaume | |
dc.contributor.author | Causse, Sebastien | |
dc.contributor.author | Szegezdi, Eva | |
dc.contributor.author | Zajonc, Dirk Michael | |
dc.contributor.author | Seigneuric, Renaud | |
dc.contributor.author | Guichard, Gilles | |
dc.contributor.author | Gharbi, Tijani | |
dc.contributor.author | Picaud, Fabien | |
dc.contributor.author | Herlem, Guillaume | |
dc.contributor.author | Garrido, Carmen | |
dc.contributor.author | Schneider, Pascal | |
dc.contributor.author | Benedict, Chris Alan | |
dc.contributor.author | Micheau, Olivier | |
dc.date.accessioned | 2018-09-20T16:06:46Z | |
dc.date.available | 2018-09-20T16:06:46Z | |
dc.date.issued | 2017-02-10 | |
dc.identifier.citation | Dufour, Florent; Rattier, Thibault; Shirley, Sarah; Picarda, Gaelle; Constantinescu, Andrei Alexandru; Morlé, Aymeric; Zakaria, Al Batoul; Marcion, Guillaume; Causse, Sebastien; Szegezdi, Eva; Zajonc, Dirk Michael; Seigneuric, Renaud; Guichard, Gilles; Gharbi, Tijani; Picaud, Fabien; Herlem, Guillaume; Garrido, Carmen; Schneider, Pascal; Benedict, Chris Alan; Micheau, Olivier (2017). N-glycosylation of mouse trail-r and human trail-r1 enhances trail-induced death. Cell Death and Differentiation 24 (3), 500-510 | |
dc.identifier.issn | 1350-9047,1476-5403 | |
dc.identifier.uri | http://hdl.handle.net/10379/11286 | |
dc.description.abstract | APO2L/TRAIL (TNF-related apoptosis-inducing ligand) induces death of tumor cells through two agonist receptors, TRAIL-R1 and TRAIL-R2. We demonstrate here that N-linked glycosylation (N-glyc) plays also an important regulatory role for TRAIL-R1-mediated and mouse TRAIL receptor (mTRAIL-R)-mediated apoptosis, but not for TRAIL-R2, which is devoid of N-glycans. Cells expressing N-glyc-defective mutants of TRAIL-R1 and mouse TRAIL-R were less sensitive to TRAIL than their wild-type counterparts. Defective apoptotic signaling by N-glyc-deficient TRAIL receptors was associated with lower TRAIL receptor aggregation and reduced DISC formation, but not with reduced TRAIL-binding affinity. Our results also indicate that TRAIL receptor N-glyc impacts immune evasion strategies. The cytomegalovirus (CMV) UL141 protein, which restricts cell-surface expression of human TRAIL death receptors, binds with significant higher affinity TRAIL-R1 lacking N-glyc, suggesting that this sugar modification may have evolved as a counterstrategy to prevent receptor inhibition by UL141. Altogether our findings demonstrate that N-glyc of TRAIL-R1 promotes TRAIL signaling and restricts virus-mediated inhibition. | |
dc.publisher | Springer Nature | |
dc.relation.ispartof | Cell Death and Differentiation | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | cancer-cells | |
dc.subject | human cytomegalovirus | |
dc.subject | carcinoma cells | |
dc.subject | receptor | |
dc.subject | apoptosis | |
dc.subject | galectin-3 | |
dc.subject | apo2l/trail | |
dc.subject | sialylation | |
dc.subject | sensitivity | |
dc.subject | induction | |
dc.title | N-glycosylation of mouse trail-r and human trail-r1 enhances trail-induced death | |
dc.type | Article | |
dc.identifier.doi | 10.1038/cdd.2016.150 | |
dc.local.publishedsource | http://www.nature.com/cdd/journal/v24/n3/pdf/cdd2016150a.pdf | |
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