dc.contributor.author | Dowling, E. P. | |
dc.contributor.author | Ronan, W. | |
dc.contributor.author | Ofek, G. | |
dc.contributor.author | Deshpande, V. S. | |
dc.contributor.author | McMeeking, R. M. | |
dc.contributor.author | Athanasiou, K. A. | |
dc.contributor.author | McGarry, J. P. | |
dc.date.accessioned | 2018-09-20T16:06:28Z | |
dc.date.available | 2018-09-20T16:06:28Z | |
dc.date.issued | 2012-07-18 | |
dc.identifier.citation | Dowling, E. P. Ronan, W.; Ofek, G.; Deshpande, V. S.; McMeeking, R. M.; Athanasiou, K. A.; McGarry, J. P. (2012). The effect of remodelling and contractility of the actin cytoskeleton on the shear resistance of single cells: a computational and experimental investigation. Journal of The Royal Society Interface 9 (77), 3469-3479 | |
dc.identifier.issn | 1742-5689,1742-5662 | |
dc.identifier.uri | http://hdl.handle.net/10379/11245 | |
dc.description.abstract | The biomechanisms that govern the response of chondrocytes to mechanical stimuli are poorly understood. In this study, a series of in vitro tests are performed, in which single chondrocytes are subjected to shear deformation by a horizontally moving probe. Dramatically different probe force-indentation curves are obtained for untreated cells and for cells in which the actin cytoskeleton has been disrupted. Untreated cells exhibit a rapid increase in force upon probe contact followed by yielding behaviour. Cells in which the contractile actin cytoskeleton was removed exhibit a linear force-indentation response. In order to investigate the mechanisms underlying this behaviour, a three-dimensional active modelling framework incorporating stress fibre (SF) remodelling and contractility is used to simulate the in vitro tests. Simulations reveal that the characteristic force-indentation curve observed for untreated chondrocytes occurs as a result of two factors: (i) yielding of SFs due to stretching of the cytoplasm near the probe and (ii) dissociation of SFs due to reduced cytoplasm tension at the front of the cell. In contrast, a passive hyperelastic model predicts a linear force-indentation curve similar to that observed for cells in which the actin cytoskeleton has been disrupted. This combined modelling-experimental study offers a novel insight into the role of the active contractility and remodelling of the actin cytoskeleton in the response of chondrocytes to mechanical loading. | |
dc.publisher | The Royal Society | |
dc.relation.ispartof | Journal of The Royal Society Interface | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | cell mechanics | |
dc.subject | actin cytoskeleton | |
dc.subject | cell contractility | |
dc.subject | chondrocyte | |
dc.subject | finite element | |
dc.subject | in vitro shear | |
dc.subject | gene-expression | |
dc.subject | micropipette aspiration | |
dc.subject | chondrocyte cytoskeleton | |
dc.subject | viscoelastic properties | |
dc.subject | mechanical compression | |
dc.subject | articular-cartilage | |
dc.subject | agarose constructs | |
dc.subject | confocal analysis | |
dc.subject | in-vitro | |
dc.subject | nucleus | |
dc.title | The effect of remodelling and contractility of the actin cytoskeleton on the shear resistance of single cells: a computational and experimental investigation | |
dc.type | Article | |
dc.identifier.doi | 10.1098/rsif.2012.0428 | |
dc.local.publishedsource | http://rsif.royalsocietypublishing.org/content/royinterface/9/77/3469.full.pdf | |
nui.item.downloads | 0 | |