dc.contributor.author | Connolly, Patrick F. | |
dc.contributor.author | Fearnhead, Howard O. | |
dc.date.accessioned | 2018-09-20T16:03:55Z | |
dc.date.available | 2018-09-20T16:03:55Z | |
dc.date.issued | 2016-10-01 | |
dc.identifier.citation | Connolly, Patrick F. Fearnhead, Howard O. (2016). Dna-pk activity is associated with caspase-dependent myogenic differentiation. The FEBS Journal 283 (19), 3626-3636 | |
dc.identifier.issn | 1742-464X | |
dc.identifier.uri | http://hdl.handle.net/10379/10880 | |
dc.description.abstract | Differentiation of myoblasts into myotubes is essential for skeletal muscle development and regeneration. Caspase-3 and caspase-9 are required for efficient myoblast differentiation. The caspase-activated endonuclease activity, CAD, and the DNA-damage repair protein XRCC1 have also been shown to be required to complete differentiation. DNA-damage associated with differentiation is accompanied by phosphorylation of Histone 2AX, an event normally catalysed by kinases ATR, ATM or DNA-PK. However, the kinase responsible for phosphorylation during differentiation is not known. Here we show that inhibition of DNA-PK, but not of ATR or ATM, blocked histone phosphorylation during differentiation. We also show that DNA-PK inhibition and siRNA-mediated DNA-PK knockdown blocked cell fusion. These data implicate a new role for DNA-PK in myogenic differentiation. | |
dc.publisher | Wiley-Blackwell | |
dc.relation.ispartof | The FEBS Journal | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | apoptosis | |
dc.subject | caspase-3 | |
dc.subject | differentiation | |
dc.subject | DNA-damage | |
dc.subject | DNA-pk | |
dc.subject | muscle | |
dc.subject | myogenesis | |
dc.subject | kinase catalytic subunit | |
dc.subject | protein-kinase | |
dc.subject | muscle differentiation | |
dc.subject | nonsense mutation | |
dc.subject | damage | |
dc.subject | gene | |
dc.subject | h2ax | |
dc.subject | identification | |
dc.subject | replication | |
dc.subject | repair | |
dc.title | Dna-pk activity is associated with caspase-dependent myogenic differentiation | |
dc.type | Article | |
dc.identifier.doi | 10.1111/febs.13832 | |
dc.local.publishedsource | http://onlinelibrary.wiley.com/doi/10.1111/febs.13832/pdf | |
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