Now showing items 1-5 of 5

    • Electrochemical activation of the natural catalytic cycle of cytochrome p450s in human liver microsomes 

      Wasalathanthri, Dhanuka P.; Malla, Spundana; Faria, Ronaldo C.; Rusling, James F. (Wiley-Blackwell, 2012-10-16)
      The natural catalytic cycle of cytochrome (cyt) P450 enzymes in human liver microsome (HLM) films was activated electrochemically via the electron transfer sequence electrode -> cyt P450 reductase (CPR)-> cyt ...
    • Electrochemiluminescence arrays for studies of metabolite-related toxicity 

      Bano, Kiran; Rusling, James F. (Wiley-Blackwell, 2016-06-01)
      This article reviews recent progress from our laboratory in electrochemiluminescence (ECL) arrays designed for screening toxicity-related chemistry of chemical and drug candidates. Cytochrome P450s and metabolic bioconjugation ...
    • Microfluidic array for simultaneous detection of dna oxidation and dna-adduct damage 

      Song, Boya; Shen, Min; Jiang, Di; Malla, Spundana; Mosa, Islam M.; Choudhary, Dharamainder; Rusling, James F. (Royal Society of Chemistry (RSC), 2016-01-01)
      Exposure to chemical pollutants and pharmaceuticals may cause health issues caused by metabolite-related toxicity. This paper reports a new microfluidic electrochemical sensor array with the ability to simultaneously detect ...
    • Screening reactive metabolites bioactivated by multiple enzyme pathways using a multiplexed microfluidic system 

      Wasalathanthri, Dhanuka P.; Faria, Ronaldo C.; Malla, Spundana; Joshi, Amit A.; Schenkman, John B.; Rusling, James F. (Royal Society of Chemistry (RSC), 2013-01-01)
      A multiplexed, microfluidic platform to detect reactive metabolites is described, and its performance is illustrated for compounds metabolized by oxidative and bioconjugation enzymes in multi-enzyme pathways to mimic natural ...
    • Voltammetric microwell array for oxidized guanosine in intact ds-dna 

      Song, Boya; Pan, Shenmin; Tang, Chi; Li, Dandan; Rusling, James F. (American Chemical Society (ACS), 2013-11-19)
      Oxidative stress in humans causes damage to biomolecules by generating reactive oxygen species (ROS). DNA can be oxidatively damaged by ROS, which may lead to carcinogenesis. Here we report a microfluidic electrochemical ...