Browsing University of Galway Support Services by Subject "polymerase chain-reaction"
Now showing items 1-5 of 5
-
Hiv type 1 mutational patterns in hiv type 1 subtype c-infected long-term survivors in karonga district malawi: further analysis and correction
(Mary Ann Liebert Inc, 2012-03-01)Here we present new sequence data from HIV-1 subtype C-infected long-term survivors (LTS) from Karonga District, Malawi. Gag and env sequence data were produced from nine individuals each of whom has been HIV-1 positive ... -
Identification of endogenous control genes for normalisation of real-time quantitative pcr data in colorectal cancer
(Springer Nature, 2010-01-01)Background: Gene expression analysis has many applications in cancer diagnosis, prognosis and therapeutic care. Relative quantification is the most widely adopted approach whereby quantification of gene expression is ... -
Identification of suitable endogenous control genes for microrna gene expression analysis in human breast cancer
(Springer Nature, 2008-01-01)The discovery of microRNAs (miRNAs) added an extra level of intricacy to the already complex system regulating gene expression. These single-stranded RNA molecules, 18-25 nucleotides in length, negatively regulate gene ... -
Microrna expression profiling to identify and validate reference genes for relative quantification in colorectal cancer
(Springer Nature, 2010-04-29)Background: Advances in high-throughput technologies and bioinformatics have transformed gene expression profiling methodologies. The results of microarray experiments are often validated using reverse transcription ... -
Multiplex pcr for identifying mycobacterial isolates.
(BMJ, 1995-03-01)Aims-To develop a multiplex polymerase chain reaction (PCR) method to facilitate identification of mycobacterial isolates. Methods-Type strains of 14 species of mycobacteria and 56 clinical isolates were lysed by boiling ...