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dc.contributor.authorDorai-Raj, Siobhanen
dc.contributor.authorO' Grady, Justinen
dc.contributor.authorColleran, Emeren
dc.date.accessioned2010-03-12T14:59:19Zen
dc.date.available2010-03-12T14:59:19Zen
dc.date.issued2009en
dc.identifier.citationDorai-Raj, S., Grady, J. O., & Colleran, E. (2009). Specificity and sensitivity evaluation of novel and existing Bacteroidales and Bifidobacteria-specific PCR assays on feces and sewage samples and their application for microbial source tracking in Ireland. Water Research, 43(19), 4980-4988.en
dc.identifier.urihttp://hdl.handle.net/10379/782en
dc.description.abstractThree novel ruminant-specific PCR assays, an existing ruminant-specific PCR assay and five existing human-specific PCR assays, which target 16S rDNA from Bacteroidales or Bifidobacteria, were evaluated. The assays were tested on DNA extracted from ruminant (n = 74), human (n = 59) and non-ruminant animal (n = 44) sewage/fecal samples collected in Ireland. The three novel PCR assays compared favourably to the existing ruminant-specific assay, exhibiting sensitivities of 91 - 100% and specificities of 95 - 100% as compared to a sensitivity of 95% and specificity of 94%, for the existing ruminant-specific assay. Of the five human-specific PCR assays, the assay targeting the Bifidobacterium catenulatum group was the most promising, exhibiting a sensitivity of 100% (with human sewage samples) and a specificity of 87%. When tested on rural water samples that were naturally contaminated by ruminant feces, the three novel PCR assays tested positive with a much greater percentage (52 - 87%) of samples than the existing ruminant-specific assay (17%). These novel ruminant-specific assays show promise for microbial source tracking and merit further field testing and specificity evaluation.en
dc.description.sponsorshipERTDIen
dc.formatapplication/pdfen
dc.language.isoenen
dc.publisherElsevieren
dc.subjectBifidobacteriaen
dc.subjectBacteroidalesen
dc.subjectMicrobial source trackingen
dc.subjectFecal pollutionen
dc.subjectPCRen
dc.subject.lcshFoodborne diseasesen
dc.subject.lcshMicrobial toxinsen
dc.subject.lcshPathogenic microorganisms -- Detectionen
dc.subject.lcshDiagnostic microbiology.en
dc.titleSpecificity and sensitivity evaluation of novel and existing Bacteroidales and Bifidobacteria-specific PCR assays on feces and sewage samples and their application for microbial source tracking in Irelanden
dc.typeArticleen
dc.local.publishedsourcehttp://dx.doi.org/10.1016/j.watres.2009.08.050en
dc.description.peer-reviewedpeer-revieweden
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