Browsing Microbiology by Author "|~|"
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An assessment of RNA content in Prymnesium parvum, Prymnesium polylepis, cf. Chattonella sp. and Karlodinium veneficum under varying environmental conditions for calibrating an RNA microarray for species detection
McCoy, Gary R.; Touzet, Nicolas; Fleming, Gerard T.; Raine, Robin (Wiley, 2014-02-03)Traditional methods of identification and enumeration can be somewhat ambiguous when identifying phytoplankton that requires electron microscopic examination to verify specific morphological features. Members of the genus ... -
Bioreactor scalability: laboratory-scale bioreactor design influences performance, ecology, and community physiology in expanded granular sludge bed bioreactors
Connelly, Stephanie; Shin, Seung Gu; Dillon, Robert J.; Ijaz, Umer Zeeshan; Quince, Christopher; Sloan, William; Collins, Gavin (Frontiers Media, 2017)Studies investigating the feasibility of new, or improved, biotechnologies, such as wastewater treatment digesters, inevitably start with laboratory-scale trials. However, it is rarely determined whether laboratory-scale ... -
Bordetella pertussis expresses a functional type III secretion system that subverts protective innate and adaptive immune responses
Boyd, Aoife (American Society for Microbiology, 2008-01)Certain bacteria use a type III secretion system (TTSS) to deliver effector proteins that interfere with cell function into host cells. While transcription of genes encoding TTSS components has been demonstrated, studies ... -
Competition between the Yops of Yersinia enterocolitica for delivery into eukaryotic cells: Role of the SycE chaperone binding domain of YopE
Boyd, Aoife (American Society for Microbiology, 2000-09)A type III secretion-translocation system allows Yersinia adhering at the surface of animal cells to deliver a cocktail of effector Yops (YopH, -O, -P, -E, -M, and -T) into the cytosol of these cells. Residues or codons 1 ... -
Contrasting responses to nutrient enrichment of prokaryotic communities collected from deep sea sites in the Southern Ocean
McCarthy, David M.; Patching, John W.; Fleming, Gerard T.A. (MDPI Open-Access Journals, 2013-09-13)Abstract: Deep water samples (ca. 4,200 m) were taken from two hydrologically-similar sites around the Crozet islands with highly contrasting surface water productivities. Site M5 was characteristic of high productivity ... -
Control of transfer and weak closure in fusion systems
Park, Sejong (2010)We show that K¿ and K¿ control transfer in every fusion system on a finite p-group when p ¿ 5, and that they control weak closure of elements in every fusion system on a finite p-group when p ¿ 3. This generalizes resultsof ... -
Engineering recombinant antibodies for polymer biofunctionalization
Hortigüela, María J.; Lucie Aumailley, Lucie; Srivastava, Akshay; Cunningham, Clare; Anandakumar, Soshee; Robin, Sylvain; Pandit, Abhay; Hu, Xuejun; Wall, Gerard (Wiley, 2015-08-18)The attachment of recognition elements such as antibody fragments to polymeric substrates can be used to mediate cell- or protein-specific interactions. In this work, single-chain Fv (scFv) antibody fragments were isolated ... -
Intracellular accumulation of high levels of gamma-aminobutyrate by Listeria monocytogenes 10403S in response to low pH: uncoupling of gamma-aminobutyrate synthesis from efflux in a chemically defined medium
Karatzas, Kimon-Andreas G.; Brennan, Orla; Heavin, Sinead; Morrissey, John J. (American Society for Microbiology, 2010-06)It is well established that the glutamate decarboxylase (GAD) system is central to the survival of Listeria monocytogenes at low pH, both in acidic foods and within the mammalian stomach. The accepted model proposes that ... -
The isolation of strains of Bacillus subtilis showing improved plasmid stability characteristics by means of selective chemostat culture
Fleming, Gerard T.; Patching, John W. (1988-03-23)A PUB 110-derived plasmid encoding chloramphenicol resistance, kanamycin resistance and high-temperature alpha-amylase showed a high degree of segregational instability when inserted into Bacillus subtilis. In an attempt ... -
LcrG is required for efficient translocation of Yersinia Yop effector proteins into eukaryotic cells
Boyd, Aoife (American Society for Microbiology, 1998-06)Extracellular Yersinia disables the immune system of its host by injecting effector Yop proteins into host cells. We show that a Yersinia enterocolitica nonpolar lcrG mutant is severely impaired in the translocation of ... -
Listeria monocytogenes has a functional gamma-aminobutyrate (GABA) shunt: Role in acid tolerance and succinate biosynthesis.
Feehily, Conor; O¿Byrne, Conor P.; Karatzas, Kimon Andreas G. (American Society for Microbiology, 2013)Listeria monocytogenes, the causative agent of human listeriosis, is known for its ability to withstand severe environmental stresses. The glutamate decarboxylase (GAD) system is one of the principal systems utilized by ... -
Low-temperature anaerobic digestion is associated with differential methanogenic protein expression
Gunnigle, Eoin; Siggins, Alma; Botting, Catherine H.; Fuszard, Matthew; O'Flaherty, Vincent; Abram, Florence (Oxford University Press, 2015-04-09)Anaerobic digestion (AD) is an attractive wastewater treatment technology, leading to the generation of recoverable biofuel (methane). Most industrial AD applications, carry excessive heating costs, however, as AD reactors ... -
Manipulation of intestinal epithelial cell function by the cell contact-dependent type III secretion systems of Vibrio parahaemolyticus
O'Boyle, Nicky; Boyd, Aoife (Frontiers Media, 2014-01-10)Vibrio parahaemolyticus elicits gastroenteritis by deploying Type III Secretion Systems (TTSS) to deliver effector proteins into epithelial cells of the human intestinal tract. The bacteria must adhere to the human cells ... -
Role of alginate lyase in cell detachment of Pseudomonas aeruginosa
Boyd, Aoife (American Society for Microbiology, 1994-07)The exopolysaccharide alginate of Pseudomonas aeruginosa was shown to be important in determining the degree of cell detachment from an agar surface. Nonmucoid strain 8822 gave rise to 50-fold more sloughed cells than ... -
The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model
Boyd, Aoife (American Society for Microbiology, 2012-03)Vibrio parahaemolyticus, a marine bacterium, is the causative agent of gastroenteritis associated with the consumption of seafood. It contains a homologue of the toxRS operon that in V. cholerae is the key regulator of ... -
The virulence plasmid of Yersinia, an anti-host genome
Boyd, Aoife (American Society for Microbiology, 1998-12)Invasive pathogenic bacteria have in common the capacity to overcome the defense mechanisms of their animal host and to proliferate in its tissues. They each have their own life-style and target organs and cause a variety ...